Mice treated with EE were delayed in estrus, metestrus
, and diestrus, typically an additional day; however, the dose of EE was not high enough to abrogate cyclicity in these mice.
Toluidine blue stain for metestrus
showing a mixture of all cells, cornified, large epithelial and neutrophils (arrow).
Cytological examination revealed that both control and vehicle mice had normal estrous cyclicity and showed different stages of the cycle containing proestrus (round, nucleated epithelial cells), estrus (cornified squamous epithelial cells), metestrus
(cornified squamous epithelial cells and predominance of leukocytes), and diestrus (predominance of leukocytes) (Table 1).
They were found in proestrus, estrus or metestrus
and were considered as reproductive females since there is hormonal stimulation for each phase and a clear indication that they are sexually receptive.
reported that the GSH content in the ovaries increased from the oestrous to metestrus
phase compared with the diestrus to pro-oestrus phase in adult rats .
SHAM Progesterone (ng/mL) 26.2 [+ or -] 4.4 * Metestrus
(12/36) 18.7 [+ or -] 5.2 Diestrus (6/36) 38.9 [+ or -] 28.3 Proestrus (9/36) 29.2 [+ or -] 14.4 Estrus (9/36) 30.3 [+ or -] 5.14 Estradiol (pg/mL) 104.8 [+ or -] 21.2 * Metestrus
(12/36) 135.8 [+ or -] 53.2 Diestrus (6/36) 57.5 [+ or -] 17.7 Proestrus (9/36) 98.6 [+ or -] 29.0 Estrus (9/36) 86.9 [+ or -] 10.9 OVX PRO + EST Progesterone (ng/mL) 6.15 [+ or -] 1.28 13.22 [+ or -] 2.93 * Estradiol (pg/mL) 42.41 [+ or -] 5.91 75.31 [+ or -] 12.82 * PRO Progesterone (ng/mL) 16.03 [+ or -] 3.38 * Estradiol (pg/mL) 35.45 [+ or -] 7.10 EST Progesterone (ng/mL) 5.68 [+ or -] 0.85 Estradiol (pg/mL) 130.61 [+ or -] 24.09 * Data are reported as means [+ or -] SE and number of SHAM rats in each estrous cycle phase.
Animals displaying the continuous microscopic image of metestrus
were recognized as pseudopregnant.
A proestrus smear primarily consisted of a predominance of nucleated epithelial cells; an estrus smear primarily consisted of anucleated cornified cells; a metestrus
smear consisted of the same proportion among leukocytes, cornified, and nucleated epithelial cells; and a diestrus smear primarily consisted of a predominance of leukocytes (MARCONDES et al., 2002).
An irregularity in cycle pattern was observed with an increase (p < 0.05) in the diestrus phase and a decrease in the proestrus (p < 0.05), metestrus
The present work shows that antagonism of NR2B subunit of NMDA receptor at the level of the ACC is able to reduce inflammatory pain in females at different phases of the estrous cycle (estrus and metestrus
) if applied before or after inflammatory insult; these results confirm that supraspinal strategies for managing pain, specifically, antagonism of NMDA receptor at the level of ACC is worthy in female at estrus and metestrus
phases of the estrous cycle.
However, two peaks periods of 17[beta]-estradiol were observed for all samples, the first in the metestrus
and the second during the proestrus (Fig.
Luteal function and reproductive response in suckled beef cows after metestrus
administration of a norgestomet implant and injection of estradiol valerate with various dosages of injectable norgestomet.