We further assessed the effect of EEAC on the migration of CL1-0 cells using the wound scratch assay in which the confluent monolayer was scraped with a sterile micropipet
tip to create a scratch wound.
and Cyclidium sp., both filter-feeding bacterivores, were isolated with a micropipet
. Single cells were passed through several washes of sterile cerophyll medium (CM: Sonneborn's Paramecium medium; Nerad 1991) to reduce gross contamination by small flagellates and bacteria, and were then transferred to fresh medium.
The surface was inoculated with 100 [[micro]liter] of a bacterial suspension using the tip of the micropipet
to spread the suspension.
- Taylor demonstrated an accurately controllable micropipet
This is done by gently drawing the oocyte into a thin micropipet
to shear off a few of the nurturing corona cells from the outer membrane.
In this study, the samples were applied to the target area of the test strip by micropipet
after 5 min.
We transferred eggs to the agarose ramps and injected them with DNA/phenol red solution using a micropipet
secured in a micromanipulator (Zeiss, Jena, Germany).
We labeled the outside surface of individual blastomeres at the two- and four-cell stages with a tiny drop of soybean oil containing 5.0 mg/ml DiI (Molecular Probes, Inc., Eugene, OR) delivered with a pressurized glass micropipet
Without disturbing the pellet at the bottom, use a micropipet
to carefully remove the clear supernatant (containing DNA) to another sterile test tube.