Microsome


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Related to Microsome: microsomal enzymes

microsome

[′mī·krə‚sōm]
(cell and molecular biology)
A fragment of the endoplasmic reticulum.
A minute granule of protoplasm.

Microsome

 

a fragment in the endoplasmic network (bubbles about 1,000 angstroms diameter), formed when cells are destroyed during the homogenization of animal and plant tissues. The microsome fraction of the homogenate is isolated by differential centrifugation. There are two types of microsomes: smooth-surfaced and rough-surfaced (as a result of the distribution of ribosomes on the latter). Before the technique of separating cellular homogenates was perfected, the microsome fraction also included mitochondria.

References in periodicals archive ?
Incubation of NPS with prepared human liver microsomes and identification human urinary metabolites by mass spectrometry is the most cost-effective technique of several available approaches.
We tested the ability of resveratrol to inhibit BNF-mediated induction of CYP1A1 and 1A2 proteins in the liver mitochondria and microsomes by Western blot analysis.
Microsomes were prepared by modification of the calcium precipitation method described by Gibson and Skett (1986).
In 1962, Sato and Omura examined the carbon monoxide-combined pigment of liver microsomes which was originally reported by Klingenberg.
They have been renamed anti-liver/kidney cytosol (LKC) because they do not react with the liver microsomes but with the cytosol and are specifically directed against the cytosolic enzyme glutathione-S-transferase theta 1 (GTT1) [6, 7].
The in vitro WEL glucuronidation activities of human liver microsomes, human kidney microsomes, human intestine microsomes, and 12 recombinant human uridine diphosphate-glucuronosyltransferase (UGT) isoforms were screened.
CYP3A4*1G genetic polymorphism influences metabolism of fentanyl in human liver microsomes in Chinese patients.
Effect of hyperthyroidism on the in vitro metabolism and covalent binding of 1, 1-dichloroethylene in rat liver microsomes. J Toxicol Environ Health 1997;52:169-88.
For each substrate, we prepared at least eight different substrate concentrations in methanol (Table 1) and further diluted them in the reaction mixture to a final volume of 100 [micro]L, containing 50 mM phosphate buffer at pH 7.4, 10 mM magnesium chloride, the specific concentration of liver or intestinal microsomes (0.1 mg/mL for BPS, 0.05 mg/mL for BPF, and 0.01 mg/mL for BPAF), and alamethicin (5% of the total protein concentration).
Genistein showed notable inhibitory effects on human (34%) and rat liver microsomes (38%) in vitro.
In vitro stability tests against mouse liver homogenates (S9 fraction) and purified mouse liver microsomes showed 66.9 [+ or -] 6.7% and 31.4 [+ or -] 7.8% intact tracer after 120 min, respectively.
Commercially available microsomes from human liver (pool of 26 men and 24 women, #HO620) and kidney (pool of 4 female and 4 male donors, #H0610.R) were purchased from Xenotech (Walkersville, MD).

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