Spectrophotometry

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spectrophotometry

[¦spek·trō·fə′täm·ə·trē]
(analytical chemistry)
A method of chemical analysis based on the absorption or attenuation by matter of electromagnetic radiation of a specified wavelength or frequency. The radiation interacts with specific features of the molecular species being determined, such as the vibrational or rotational motions of the chemical bonds. The radiation can also interact with specific atoms or the whole molecule, for example, by causing the molecule to change its electronic energy state.

Spectrophotometry

 

a branch of measurement technology that draws on spectrometry, photometry, and metrology in developing methods and instruments for the quantitative measurement of spectral absorptance, reflectance, emissivity, and luminance as quantities characterizing media, coatings, surfaces, and radiators.

References in periodicals archive ?
Comparison and identification of automotive topcoats: Microchemical spot tests, microspectrophotometry, pyrolysis-gas chromatography, and diamond anvil cell FTIR, Canadian Society of Forensic Science Journal (1994) 27:209-223.
Microspectrophotometry and colorimetry are two analytical techniques for obtaining an objective color measurement.
the Bobolink, a migratory songbird.--Using microspectrophotometry we
These included radioactive isotopes, electrophoresis, microspectrophotometry, the electroencephalogram, and electromyogram.
Zeiss has always been a leader in microspectrophotometry, and its recently reconfigured product line sets new benchmarks, as is demonstrated by its modularity and flexibility.
corniculatus photoreceptors, as revealed by microspectrophotometry (MSP).
Once the samples are classified with reference to their probable source, a detailed study including microspectrophotometry and elemental analysis (Choudhry 1991) can be performed for a conclusive opinion.
UV absorption microspectrophotometry and digestibility of cell types of bermudagrass internodes at different stages of maturity.
These studies of the organization and dynamics of the root meristem are based on several lines of evidence, including observations on apical meristem cytohistological zonation, ultrastructure, cell cycle durations, mitotic indices, DNA microspectrophotometry, incorporation of radioactively labeled DNA precursors, and interpretations of cell lineages.
(2013) identified a surprisingly large number of opsin transcripts in the eyes of stomatopods--up to 33 transcripts--that far outnumber physiologically distinct photoreceptor classes determined by microspectrophotometry or electrophysiology.
Microspectrophotometry provides information about the ink's acting as a chemical mixture, but still does not provide the necessary detail that is important in component analysis for ink differentiation (Zeichner et al.