Many empty spaces are observed in the cytoplasm of these cells, due to the preparation for microtomy
that removed granules present in the cells (arrows in Figure 4A, B).
This gave a total of four scantlings from each disc that were further cut into 0.02-[m.sup.2] cubes and prepared for maceration and microtomy
To this end, a novel microtoming method, ultra-low-angle microtomy
(ULAM), has been developed that can resolve material composition as a function of depth.
of the reproductive organs was performed using the method of Prophet et al.
The specimens were fixed on paraffin wax and blocks prepared for microtomy
. After fixation vertical sections of the skin were cut with the help of semiautomatic rotary microtome (Remi-1871, India).
: A sample preparation method that sequentially passes a blade at a shallow depth through a sample, resulting in sections of selected thickness as well as a flat block.
Particular attention should be given to preimaging steps such as tissue processing and microtomy
to minimize artifacts known to adversely affect image quality.
The blocks were submitted to microtomy
in an automatic microtome (Leica, RM2165, Germany), obtaining cuts of 5 mm, which were adhered in histological slides and left in an oven at 60 [degrees]C.
After step-wise tissue processing, embedding, blocking and microtomy
of the available core needle biopsy specimens, haematoxylin and eosin (H and E) stained sections were prepared for light microscopic examination in each case.
Tumor sections (5 [micro]m thick) were obtained by microtomy
and de-paraffinized using xylene and rehydrated in a graded series of ethanol and finally in distilled water.
The powder was embedded in an epoxy resin (polaron 612) before microtomy
Serial sections from each specimen were obtained by microtomy
and stained by the Mallory's Trichrome method (Bancroft and Stevens, 1996) Serial sections were qualitatively evaluated for the presence of fibrin, using a computer image acquisition system (Carl Zeiss microscope Axioscop 2 plus Axiocam camera).