[1,2] However, study done by Nikolic S, Mictic
J et al found much higher ratio of male to female of 3.17:1.
Restriction sites BamH I and EcoR I were used to fuse BnPIP-like gene fragment into vector pPIC3.5K with mictic
tag at N-terminal and His tag at C-terminal (Fig.
In response to some environmental factors, daphnids start sexual reproduction; males appear and females start producing mictic
eggs, which will be fertilized by the males.
Males and mictic
females occurred when densities were high.
Sexuality is initiated by an environmental stimulus that induces amictic (female-producing) females to produce mictic
Our electrophoretic and cytological observations provide direct evidence that triploid production occurs by addition of the fertilizing male genome, and also support the hypothesis that the mictic
phase of egg maturation of B.
Only amictic females (asexually reproducing) were included in the experiments; mictic
females (females producing males or sexually produced resting eggs) were discarded.
This strongly suggests that smausdd is indeed mictic
, although it will take genetic analysis of [F.sub.1] offspring to confirm this.
Any females later proving to be mictic
instead of amictic females were discarded.
If this is true, then the data would lend support to the notion that truly asexual taxa possess general-purpose genotypes even in a limited comparative situation (i.e., compared with close relatives that have both mictic
and amictic phases in their life cycle).