ProExC, a novel immunohistochemical cocktail--composed of topoisomerase II a (TOP2A) and minichromosome
maintenance protein 2 (MCM2) antibodies--has also been recently suggested to be a useful marker of high-grade dysplasia (25,26): strong nuclear staining in more than half of the epithelial thickness showed 90% sensitivity and 79% positive predictive value for HSIL.
Three types of B chromosomes were identified: a subtelocentric B (BST), a metacentric B chromosome (BT) and a small B minichromosome
maintenance proteins 2 and 5 in non-benign epithelial ovarian tumours: relationship with cell cycle regulators and prognostic implications.
The primer ser for each gene was followed: histone deacetylase 9 (HDAC9) forward, 5'-GCAAGAGGAGACAGAGACCG-3'; HDAC9 reverse, 5' - ACTTG G C ACTTCAC AAG G CT- 3'; minichromosome
maintenance deficient 3 associated protein (MCM3AP) forward, 5'-CGCTTCCTCTGGTGGTTCTT-3'; MCM3AP reverse, 5'CTGCACTGCTTGCAAAACCT-3'; GAPDH forward, 5'-TCACCCACACTGTGCCCATCTATGA-3'; GAPDH reverse, 5'-GAGGAAGAGGATGCGGCAGTGG-3'.
The MCM-2 protein belongs to the minichromosome
maintenance protein complex family and plays an important role in the two stages of the cell cycle: replication of DNA and cell division.
Binding of Human Minichromosome
Maintenance Proteins with Histone H3," Journal of Biological Chemistry 271 (1996): 24115-22.
Conversion of viral genome to a covalently closed circular DNA (cccDNA), which is formed in the nuclei of infected hepatocytes within the first 24h following virus inoculation and forming of a minichromosome
after binding to proteins, is the molecular basis of persistence13.
In prostate cancer, results of a recently reported study demonstrated that binding of the vitamin D (1,25-dihydroxyvitamin D3) receptor [encoded by the vitamin D (1,25-dihydroxyvitamin D3) receptor (VDR) gene] to the MCM7 promoter [encoded by minichromosome
maintenance complex component 7 (MCM7)] resulted in acetylation of histone H3 lysine 9 and, subsequently, enhanced transcription of miR-106b, which is located in an intron of the MCM7 gene (25).
Proteins encoded by the minichromosome
maintenance (MCM) genes have also been proposed as useful markers of proliferation; with high levels of gene expression indicating poor prognosis (20).
Comparative Analysis of Cervical Cancer in Women and in a Human Papillomavirus-Transgenic Mouse Model: Identification of Minichromosome
Maintenance Protein 7 as an Informative Biomarker for Human Cervical Cancer.
The best studied example of colonocyte testing in feces is the minichromosome
maintenance proteins (MCM).
Justin Davies described a novel test that utilizes antibodies to detect minichromosome
maintenance protein 2 (Mcm2) in colonocytes exfoliated onto the surface of stool.