"On the whole, Cas12a is better, but there were some areas where Cas12a was still surprisingly blind to some mispairing
between its RNA and the genomic target," Finkelstein said.
smMIP-mediated error correction substantially decreased the number of artefactual microsatellite alleles resulting from slipped strand mispairing
during PCR amplification ("stutter" artifact) (28), enabling the identification of true allele lengths (Fig.
occidentalis genome (v1.0) with TopHat2 (allowing two bases of mispairing
and multiple hits < 20) and then assembled by using the Cufflinks suite with default parameters.
8-Oxoguanine is the most common and mutagenic oxidized DNA lesion and is repaired by OGG1 to avoid mutagenic base mispairing
. However, oxidatively generated DNA lesions are not only a mutagenic risk factor but also a regulatory marker (for a review, see ).
When DNA is alkylated for instance in guanine, apurinic sites could be created which could induce mispairing
of nucleotide bases.
This length variation is thought to occur through slipped strand mispairing
during replication (Densmore et al.
Because BaP mainly induces G [right arrow] T transversions via mispairing
of adducted DNA (Keohavong and Thilly 1992), increased proportions of G^T (as well as G [right arrow] C transversions and deletions) were observed (Figure 1B).
Recently, TDG was also shown to be involved in T mispairing
with several types of exocyclic etheno-base lesions, such as [epsilon]C .
Several reasons may account for discordant interpretations when using telemicrobiology for the remote analysis of Gram stains, all of which occurred in the McLaughlin et al (14) study: (1) poor field selection by the photographer, which decreased the sensitivity of the interpretation; (2) poor image quality, which compromised the accuracy of interpretation; (3) too few fields photographed, which resulted in diagnostic uncertainty by the interpreter; and (4) inappropriate case identification by the interpreter, which resulted in mispairing
of the case number and its interpretation.
They are hyper variable in length (Tautz, 1989) as a result of DNA-replication errors, such as slipped strand mispairing
(Strand et al., 1993).
These amplicons generate some cross-hybridized products with a resulting single-base mispairing
in their middle, and generally melt slightly before either of the homozygous products).