We also uncovered an impact of Nesprin-2 on mitotic chromosomes
that might be mediated by an interaction with the condensin core units SMC2/4.
The most frequent haploid number found in 50 metaphase I oocytes was n = 19 mitotic chromosomes
However, it is not clear whether pRB directly interacts with HP1[alpha] to repress the expression of E2F-target genes because HP1[alpha] was found to be preferentially phosphorylated in the G2/M phase and to bind to histone H3 modified with both K9me3 and phosphorylated serine 10 in mitotic chromosomes
were performed according to the technique "air drying" as modified for fish by Bertollo et al.
Karyotype and nor-banding of mitotic chromosomes
of some Vitis L.
Bookmarking genes for activation in condensed mitotic chromosomes
. BioEssays 20:275279.
were counted in blastomeres of 32-cell embryos.
Preparation of mitotic chromosomes
of leaf-cutting ants from the genera Atta and Acromyrmex.
During the mitotic phase of the cell cycle, a partial condensation of the DNA occurs in prophase, so that the mitotic chromosomes
occupy a much smaller volume of the nucleus.
To obtain mitotic chromosomes
in vitro, portions of anterior kidney were removed and transferred to RPMI medium (Cultilab) with about 50 [micro]L of 0.025% colchicine, followed by hypotonic treatment (KCL 0.075 M) for 20 minutes at 37[degrees]C and fixation in Carnoy's fixative (methanol: acetic acid 3 : 1) .
Using lentiviral particles also allows effective direct visualization of Nuclei, Nuclear Membrane, Mitotic Chromosomes
and Interphase Chromatin, Endosome, Endoplasmic Reticulum, Microtubule, Mitochondria, Golgi, Lysosome, Plasma Membrane, Cytoplasm and Peroxisome in hard to transfect mammalian cells, stem cells and primary cells.
However, there are no available reports on karyotypes obtained from gill tissue of freshwater crayfish although the analysis of mitotic chromosomes
provides a good tool to obtain the modal diploid chromosome number.