Okugawa et al., "In vivo optical imaging of cancer metastasis using multiphoton microscopy
: A short review," American Journal of Translational Research, vol.
Webb, "Nonlinear magic: multiphoton microscopy
in the biosciences," Nature Biotechnology, vol.
Advances in multiphoton microscopy
for imaging embryos.
also yields high-resolution images, but with even higher axial resolution, greater sample penetration, reduced photobleaching of marker dyes and increased cell viability.
Rubin notes that Thomas Bifano, at Boston University, has shown that combining multiphoton microscopy
with AO can also make it easier to study the Brain .
(MPM) is a nonlinear, laser-based imaging technique that can generate high-resolution "histology-quality" images in real time without the need for any special tissue processing or exogenous dyes.
Simultaneous multiple-excitation multiphoton microscopy
yields increased imaging sensitivity and specificity.
Analysis of 3D images has also become essential with the increasing use of confocal and multiphoton microscopy
. Tile View, Slice View and Volume View have all been enhanced with extra features for improved viewing and manipulation.
The Chameleon line is the only series of one-box, ultra-fast lasers designed specifically to maximize the potential of multiphoton microscopy
, including CARS and three-photon imaging.
In its report on the acquisition, the Federal Cartel noted Leica's concerns about a Zeiss monopoly on multiphoton microscopy
. Carl Zeiss had no comment on the report and Bio-Rad could not be reached for comment.
These advances deliver improved reliability, lower operating costs, and simplified integration into optically complex applications such as multiphoton microscopy
. The pump laser is a diode-pumped solid-state laser that uses only one pump diode.
These techniques include full-field optical coherence tomography, confocal fluorescence microscopy (CFM), and multiphoton microscopy
. (8-12) These optical imaging modalities have been widely used for ex vivo imaging in research studies, but there is limited evidence regarding the feasibility of their use in routine surgical pathology practice.