Caption: Figure 3: Infection of primary human retinal pigment epithelial cells and primary human retinal endothelial cells with DENV: viral strain = Mon601; multiplicity of infection
=1; evaluated time point postinoculation = 48 hours.
The neurons were infected with Toro-2003 or HB171/11 strain with 0.001 multiplicity of infection
at day 7 postseeding, and viral growth was determined at indicated time points by focus forming assay, as previously described (15).
Abbreviations ALT: Alanine aminotransferase AST: Aspartate aminotransferase i.v: Intravenous IL-33: Interleukin 33 KO: Knockout LL: Liver leukocytes MHV: Mouse hepatitis virus MOI: Multiplicity of infection
PI: Post infection PMH: Primary mouse hepatocytes.
To test the antiviral activity of Bioaron C[R] during virus replication (therapeutic protocol), cells (MDCK, HEp-2, HeLa, BGM) were infected with a multiplicity of infection
(M.O.i.) as described above.
Abbreviations [C.sub.q]: Quantitation cycle cRNA: Antigenomic RNA CV: Coefficient of variation F: Fusion protein gRNA: Genomic RNA HN: Haemagglutinin-neuraminidase L: Large protein M: Matrix protein MOI: Multiplicity of infection
mRNA: Messenger RNA ND: Newcastle disease NDV: Newcastle disease virus NP: Nucleoprotein P: Phosphoprotein PBS: Phosphate-buffered saline pi: postinfection qPCR: Quantitative PCR RT: Reverse transcription RT-PCR: Reverse transcription polymerase chain reaction SPF: Specific pathogen-free [TCID.sub.50]: 50% tissue culture infection dose vRdRp: Viral RNA-dependent RNA polymerase vRNA: Viral RNA.
The differentiated MDMs were infected with S7, S10, H37Rv or H37Ra at a multiplicity of infection
(MOI) of 1:10 for 3 h.
pestis cells/mL and a multiplicity of infection
(MOI) of 100 on the basis of OD 600 calculations.
HT29 cells were infected with the different EHEC strains at a multiplicity of infection
(MOI) of 100 for the specified time points.
Before infection of MSC, the virus-containing solution was thawed and diluted in tryptophan-free RPMI 1640 medium to reach a multiplicity of infection
(MOI) of 0.1-10.
On day 2 after seeding, neonatal microglial cultures were infected at a multiplicity of infection
(MOI) of 2: 1 with RSA59 or mock-infected with noninfected cell lysate.
Monolayers of Vero and C6/36 cells seeded onto 24-well plates were infected with DENV-2 at a multiplicity of infection
(MOI) of 0.1.
Canine, human, feline, and chicken cells were infected at a multiplicity of infection
of 0.005 at temperatures mimicking those of the upper and lower respiratory tract of the respective species (i.e., 37[degrees]C and 39[degrees]C for chicken cells; 33[degrees]C and 37[degrees]C for the remaining cells) (Figure 2).