Because Gn and Gc are major immunogens of orthobunyaviruses (10), the mutation hot spot
was supposed to be involved in immune evasion mechanisms and/or adaption of the cell tropism within the individual host (7,9).
The only CFTR mutation hot spot
was in codon 556 (Ile556Val), and of the 13 patients with this substitution, 12 had HLP.
Mutation hot spot
for nonspecific X-linked mental retardation in the MECP2 gene causes the PPM-syndrome.
Based on the findings from these patients, they then performed targeted sequencing of suspected mutation hot spots
in another 71 patients.
P53 gene mutation had many forms and multiple mutation hot spots
, but most of the mutation types were G-A and T-C.
For such mutation hot spots
, pyrosequencing provides the possibility to design a nucleotide dispensation order that increases the sensitivity of detection in combination with identification of all the relevant mutation variants.