Repeat bone marrow biopsy and aspirate (Figure 1(a)) showed 64% myeloblasts
which expressed a slightly different immunophenotype than originally (CD13, CD33, CD15, CD58, CD117, and HLA-DR).
Consecutive phases vary, giving rise to a kinetically distinct mitotic pool in which numbers expand to include the myeloblasts
, progranulocytes and myelocytes - loosely referred to as the fixed components of the microenvironment.
On special staining we found Myeloblasts
of AML and CML showed positive (coarse brown black granules) staining with MPO in more than 3% of blasts in all cases except one in which NSE stain was positive.
Leukocytosis is invariably present at diagnosis with less than 5% myeloblasts
and varying numbers of promyelocytes, myelocytes, metamyelocytes, basophils, and neutrophils (Slide 1).
Blastic type is composed predominantly of myeloblasts
, immature type is composed of myeloblasts
and promyelocytes, differentiated type is composed of more promyelocytes and later stages of maturation(8).
Blasts look like either lymphoblasts or myeloblasts
and present as monomorphic medium-sized cells with irregular nuclei, one or more prominent nucleoli, and scant cytoplasms.
Clinical and laboratory features of seven patients with acute myeloid leukemia (AML)-M2/M3 and elevated myeloblasts
and abnormal promyelocytes.
Acute Myeloid Leukemia, the most common acute leukemia (blood and bone marrow cancer) in adults, is characterized by the proliferation of abnormal myeloblasts
(a type of white blood cell) in the bone marrow.
A type of cancer in which the bone marrow makes abnormal myeloblasts
, red blood cells or platelets, it is a slowly progressing disease that manifests itself in middle-aged people, rarely afflicting children.
BM Aspirate from a patient with Acute Myeloid Leukaemia showing Myeloblasts
. Leishman Stain x40
Further, 42% of his bone marrow cells were myeloblasts
with folded nuclei, prominent nucleoli, narrow cytoplasm, no granules, and no Auer rods.
The WBC precursor channel (WPC), one of the new channels, is based on fluorescence flow cytometry technology and was developed particularly to detect myeloblasts
or lymphoblasts more accurately than the immature myeloid information channel used in the XE series.