myeloblastosis


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myeloblastosis

[‚mī·ə·lō·bla′stō·səs]
(medicine)
Diffuse proliferation of myeloblasts, with involvement of blood, bone marrow, and other tissues and organs.
References in periodicals archive ?
As discussed in the introduction and in our recent review, (4) the Abbott Molecular 4-probe melanoma FISH test targeting ras responsive element binding protein 1 (RREB1, 6p25), myeloblastosis viral oncogene homologue (MYB, 6q23), centromere 6, and cyclin D1 (CCND1,11q13) probes5 is the first commercially available FISH probe set showing promise as a diagnostic adjunct in evaluation of melanocytic lesions.
CCND1, cyclin D1;CEN6, centromere 6; MYB, v-myb myeloblastosis virus oncogene homologue;RREB1, ras responsive element binding protein 1.
34) RTPCR was performed using a Takara RNA PCR kit (Avian Myeloblastosis Virus version 2.
The reaction was performed by PTC-200 programmable thermocycler (MJ research, USA) in a 20 [micro]l mixture consisting of 15 U avian myeloblastosis virus (AMV) reverse transcriptase, 20 U of RNasin ribonuclease inhibitor, 25 ng random primers, 5 mM MgCl2, 1 mM deoxynucleoside triphosphate (dNTP) mix and 1X reverse transcription buffer (10 mM Tris-HCl at pH 8.
Total RNA was extracted from rodent lung tissues by using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions and subjected to RT-PCR for amplification of partial hantavirus small (S) segment sequences, cDNA was synthesized with avian myeloblastosis virus reverse transcriptase (Promega, Beijing, China) in the presence of primer P14 (20).
T]SL2, cathepsin L2; ESR1, estrogen receptor 1; GRB7, growth factor receptor-bound protein 7; GSTM1, glutathione S-transferase M1; ERBB2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); MKI67, antigen identified by monoclonal antibody Ki-67; MYBL2, v-myb myeloblastosis viral oncogene homolog (avian)-like 2; PGR, progesterone receptor; AURKA, aurora kinase A; MMP11, matrix metallopeptidase 11 (stromelysin 3); BIRC5, baculoviral IAP repeat-containing 5 (survivin); ACTB, actin, beta; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GUSB, glucuronidase, beta; KPLPO, ribosomal protein, large, P0; TFRC, transferrin receptor (p90, CD71).
2 [micro]g total RNA was reversely transcribed by incubation at 42[degrees]C for 1 h in a 20 [micro]l mixture consisting of 10 U avian myeloblastosis virus reverse transcriptase, 20 U RNase inhibitor, 5 [micro]mol random primers, 50 mmol/L tris-HCl (pH 8.
Reverse transcriptase from avian myeloblastosis virus (Promega, Madison, WI, USA) was used in the first round RT-PCR.
MMLVH; Promega); MMLV (Promega); avian myeloblastosis virus (AMV; Promega); Improm-II (Promega); Omniscript (Qiagen); cloned AMV (cAMV; Invitrogen); ThermoScript RNase [H.
The reactions require avian myeloblastosis virus reverse transcriptase, ribonuclease H, T7 RNA polymerase in the master mix, and a thermostatic fluorimeter; the probe of choice is the Molecular Beacon.