myeloblastosis


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myeloblastosis

[‚mī·ə·lō·bla′stō·səs]
(medicine)
Diffuse proliferation of myeloblasts, with involvement of blood, bone marrow, and other tissues and organs.
References in periodicals archive ?
As discussed in the introduction and in our recent review, (4) the Abbott Molecular 4-probe melanoma FISH test targeting ras responsive element binding protein 1 (RREB1, 6p25), myeloblastosis viral oncogene homologue (MYB, 6q23), centromere 6, and cyclin D1 (CCND1,11q13) probes5 is the first commercially available FISH probe set showing promise as a diagnostic adjunct in evaluation of melanocytic lesions.
Total RNA was extracted (TRIzol reagent; Gibco BRL, Grand Island, NY, USA), treated with DNase 1 (Takara Bio Inc., Kyoto, Japan), and reverse transcribed into single-stranded cDNA by using random hexamers (First Strand cDNA Synthesis Kit for reverse transcription PCR (RT-PCR) [avian myeloblastosis virus]); Roche Diagnostics, Indianapolis, IN, USA).
The quantity and quality of the isolated RNA were determined by biophotometer (Eppendorf, Hamburg, Germany) and agarose gel electrophoresis, respectively, and RT was carried out using RT reactions (10 ml) consisting of 500 ng of total RNA, 5 mmol/L Mg[Cl.sub.2], 1 ml RT buffer, 1 mmol/L dNTP, 2.5 U avian myeloblastosis virus reverse transcriptase, 0.7 nmol/L oligo d(T), and 10 U ribonuclease inhibitor (TaKaRa Biotechnology, Co., Ltd, Dalian, People's Republic of China).
One microgram of DNase I-treated RNA was reverse transcribed with avian myeloblastosis virus reverse transcriptase (USB Corp., Cleveland, OH, USA) using dT20 primers and random hexamers, and was ultimately resuspended in 100 [micro]L Tris-EDTA buffer.
Total RNA obtained from each sample (1 [micro]g) was reverse-transcribed (RT) using oligo-(dT)15 primers and avian myeloblastosis virus (AMV) reverse transcriptase (Promega, Madison, WI, USA).
NASBA uses avian myeloblastosis virus reverse transcriptase (RT), RNase H, and T7 bacteriophage RNA polymerase, whereas TMA uses an RT enzyme with endogenous RNase H activity and T7 RNA polymerase.
The avian myeloblastosis virus (AMV) reverse transcriptase, Taq DNA polymerase and other related reagents were products of Promega, USA.
This panel consisted of ras responsive element binding protein 1 (RREB1, 6p25), v-myb myeloblastosis viral oncogene homologue (MYB, 6q23), CEN6 (centromere 6), and cyclin D1 (CCND1, 11q13) probes (18) (Figure 1, B).
cDNA was synthesized by using avian myeloblastosis virus reverse transcriptase (Promega Corp., Madison, WI, USA) and downstream consensus primer (D2) for DENV and random hexamers for CHIKV.
CCNE1 and v-myb myeloblastosis viral oncogene homolog avian-like 2 (MFBL2) are important genes involved in the [G.sub.1]--S transition and are transcriptional targets of E2F (Yasui et al.
An aliquot of 8.2 [micro]L standardized to 1 [micro]g of RNA was reverse transcribed with avian myeloblastosis virus-reverse transcriptase (AMV-RT) and [oligo-p(dT).sub.15]-primer following manufacture's instruction (1st Strand cDNA Synthesis Kit, Roche, Mannheim, Germany).
(34) RTPCR was performed using a Takara RNA PCR kit (Avian Myeloblastosis Virus version 2.1, Takara Inc Ltd, Dalian Branch, Dalian, China) under the following conditions: 0.8 mg of total RNA was reverse-transcribed at 55[degrees]C for 30 minutes in a 20-[micro]L solution containing random primer, and the reaction was terminated by incubating at 99[degrees]C for 5 minutes.