Maximal effects and logEC50s [means [+ or -] standard deviation (SD)] from myograph
analyses of endothelial function following 4 mo of filtered air (FA) or concentrated ambient particulate matter [(PM).sub.2.5] (CAP) exposure in male [Nestin-creIKK2.sup.flox/+] (control) and [Nestin-creIKK2.sup.flox/flox] ([IKK2.sup.Neu-KO]) mice at 8-12 weeks of age (n = 6-7/group).
Muscle endurance was measured as fatigue resistance using the same myograph
and set up as for the measurement of strength.
Mechanical activity was recorded isometrically using a wire myograph
(DMT, Aarhus, Denmark).
The isolated esophagus was cut transversally to produce ring-like segments (approximately 2-mm length) that were mounted horizontally onto a wire myograph
(610M-DMT; DMT, Aarhus, Denmark).
Abdominal aortic rings were mounted in myograph
chambers, where they were maintained in Krebs' solution at 37[degrees]C, continuously supplied with carbogen (95% C[O.sub.2], 5%[O.sub.2]).
The uterus was removed, uterine arcuate arteries dissected free from surrounding tissue and either mounted on a small vessel wire myograph
or frozen in liquid nitrogen until analysis.
Vessel rings were then mounted on a wire myograph
(Danish MyoTechnology, Aarhus, Denmark) filled with physiological salt solution (PSS) as described previously [19, 26, 27].
The experiments were performed with a DMT 610M Multi Chamber Myograph
connected to an ADlnstruments PowerLab 4/30 signal transducer and to a Grass S48 Electrical field stimulator.
After removal of the surrounding connective tissue, the arteries were cut into 4-6 ring segments of ~1.6mm length for functional assessment of endothelial function in a Multi Myograph
System (610M, Danish Myo Technology A/S, Aarhus N, Denmark).
Aortic rings of 2-3 mm in width were mounted to organ baths (Myograph
601, Danish Myo Technology, Aarhus, Denmark.
Immediately after scarification, the mouse aortas were extracted, cut into pieces as aortic rings, and mounted in a Wire Myograph
System (610M, Danish Myo Technology, Aarhus, Denmark) using two parallel L-shaped metal prongs that connected with a force transducer.