negative staining

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negative staining

[′neg·əd·iv ′stān·iŋ]
(biology)
A method in microscopy for demonstrating the form of cells, bacteria, and other small objects by staining the ground rather than the objects.
References in periodicals archive ?
This finding was quickly corroborated by negative stain EM, IHC assay, serologic testing, and molecular assays.
Negative stain EM showed that the helical nucleocapsids have a herringbone appearance and an average diameter of 21 nm.
16) Negative stain electron microscopy is a useful method for addressing questions concerning size distribution of the liposome, and it is a reliable technique that is simple to perform and requires only limited specialized equipment.
1A), the use of different negative stains revealed different appearances (Fig.
The comparison of different negative stains revealed that all tested stains are, in principle, suitable for EM detection of polyomavirus and that a positive result is not dependent on the stain chosen.
A coated grid with sample adsorbed to the surface is floated on a drop of negative stain for 0.
Several major pitfalls exist in the identification of viral agents by negative stain electron microscopy.
Immunohistochemical analysis for p53 protein was performed on several sections (dilution 1:50) and revealed a spectrum of tumor nucleus--positive staining from 0 to 4+, with the majority of nuclei showing 2+ to 4+ localization of this antigen (Figure 1, C) and negative stain on the negative control.
With negative stains, the report reads: "No herpes simplex isolated.
A second needle biopsy sample showed chronic inflammation with a histiocytic reaction and negative stains for AFB and fungi, but it was deemed nondiagnostic.
Despite negative stains and cultures, the identification of DNA from M.
Negative stains included c-kit, placental alkaline phosphatase, epithelial membrane antigen, and [alpha]-fetoprotein.