negative staining


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negative staining

[′neg·əd·iv ′stān·iŋ]
(biology)
A method in microscopy for demonstrating the form of cells, bacteria, and other small objects by staining the ground rather than the objects.
References in periodicals archive ?
241) However, desmoid tumors typically show nuclear [beta]-catenin staining, which is absent in GISTs, and negative staining for DOG1.
In the present study, only two cases of dysgerminoma showed negative staining for OCT-4.
1) to compare sensitivity of negative staining techniques using various stains such as malachite green, crystal violet, and methylene blue for rapid detection of oocysts of Cryptosporidium, Cyclospora, and Cystoisospora;
One case diagnosed as T-cell lymphoblastic lymphoma which showed positivity for CD 99 but showed negative staining to vimentin hence the possibility of EWS/PNET was eliminated.
Negative staining theoretically replaces water in the interstices of the object until all hydrated volumes are filled with stain, thus forming a uniformly thin, amorphous film in which the specimen is supported and preserved.
Figure 4 is the digital histogram interpretation of P2 population (see Figure 2) showing negative staining of all events.
In addition, negative staining can also provide information on whether liposomes produced in a particular manner are multi- or unilamellar.
Electron microscopy with negative staining of aqueous lysates of the affected skin and of bleeding quills showed isolated or clustered polyomavirus particles 45-50 nm in size.
We did this by evaluating different EM preparation techniques [standard negative staining, ultracentrifugation (UC) before EM, and immune-specific particle enrichment] and then comparing the best of these EM assays with an up-to-date real-time PCR assay on 531 clinical samples.