negative staining

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negative staining

[′neg·əd·iv ′stān·iŋ]

(biology)

A method in microscopy for demonstrating the form of cells, bacteria, and other small objects by staining the ground rather than the objects.

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For the enrichment of virus particles from urine, samples were subjected to UC before negative staining and the results were compared with those obtained with direct EM, i.

Detection of human polyomaviruses in urine from bone marrow transplant patients: comparison of electron microscopy with PCR

Since some of the stain settled into furrows on top of the unexpected shapes, the negative staining revealed fine details of the exterior of these mysterious interlopers, including arches that reminded Rome and Kedersha of the ceilings in medieval cathedrals.

What's in the vault: an ignored cell component may often account for why chemotherapy fails

One case was entirely removed (cytology and surgical specimens) from the study after it was found to be a metastatic pulmonary nodule in a patient with colonic ACA with negative staining.

Tissue-preserving antibody cocktails to differentiate primary squamous cell carcinoma, adenocarcinoma, and small cell carcinoma of lung

With the introduction of negative staining in the late 1950s (12) and the wider availability of electron microscopes, electron microscopy (as a catchall method) became essential in characterizing many new isolates detected in diagnostic cell cultures and clinical samples, e.

Electron microscopy for rapid diagnosis of infectious agents in emergent situations (1). (Synopses)

This reaction mixture was provided as a sample for negative staining.

Dynamic reaction in a homogeneous HDL-cholesterol assay visualized by electron microscopy

These features (including negative staining on a frozen section stained with oil red O) were present in a case of silicone pneumonitis that we reviewed resulting from leakage of silicone gel from a breast prosthesis (Figures 1-4).

Detection of silicone in lung tissue

Careful morphologic examination along with HMB-45 positive staining and negative staining for epithelial markers, like pancytokeratin, can help to successfully differentiate E-AML from clear cell RCC or other primary renal neoplasms, which do not stain for HMB-45 and are positive for epithelial markers.

Surgeon-controlled robotic partial nephrectomy for a rare renal epithelioid angiomyolipoma using near-infrared fluorescence imaging using indocyanine green dye: A case report and literature review

The diagnosis of seminoma was confirmed by further immunohistochemical studies (figure 2, C), although the negative staining for placental alkaline phosphatase and CD 117 are considered to be atypical for seminoma.

Metastatic seminoma with cervical lymphadenopathy as the initial manifestation

Double staining for maspin and pVHL in DAC demonstrated the same result: positive staining for maspin and concurrent negative staining for pVHL.

Reevaluation and identification of the best immunohistochemical panel (pVHL, maspin, S100P, IMP-3) for ductal adenocarcinoma of the pancreas

A: Photomicrograph showing atypical spindle cells with marked pleomorphism, and frequent mitotic figures (H&E, x40); B: Immunohistochemical stain showing negative staining for AFP (x60); C: Immunohistochemical stain showing negative staining for cam 5.

A unique case of a sarcoma arising in a testicular non-seminomatous mixed germ cell tumour with a predominant yolk sac component

We analyzed the presence of flagella by negative staining and transmission electron microscopy.

Characterization of flagella produced by clinical strains of stenotrophomonas maltophilia

Desmin, S-100 protein, chromogranin, synaptophysin, somatostatin, and factor VIII showed negative staining within tumor cells.