negative staining


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negative staining

[′neg·əd·iv ′stān·iŋ]
(biology)
A method in microscopy for demonstrating the form of cells, bacteria, and other small objects by staining the ground rather than the objects.
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Caption: Figure 2: (a) Photomicrograph immunohistochemical ([IHC] x400) showing malignant cells with negative staining for CD-45.
Immunohistochemical stain on cell block showing negative staining for Thyroglobulin (Thyroglobulin stain X 200).
Immunohistochemical stains for basal cells, such as HMWCK or more recently p63 are currently used in the diagnosis of focal prostate cancer but there are limitations in their use as negative staining for basal cell markers is by itself not diagnostic of carcinoma and also due to technical reasons these stains may be falsely positive thus it is necessary to interpret negative staining in an atypical focus in light of positive staining in adjacent benign glands which is used as an internal positive control.
No membrane debris, observed by electron microscopy using negative staining confirmed the purity of OMVs from both strains.
[37.] Rodriguez C, Hardy E (2015) Lipopolysaccharide aggregates in native agarose gels detected by reversible negative staining with imidazole and zinc salts.
Histological examination of a renal leiomyoma reveals fusiform to spindle shaped cells with positive immunohistochemical staining for smooth muscle markers (desmin, caldesmon, and SMA) and negative staining for HMB-45 and cathepsin K.
Negative staining for lymphatic marker, (D) D2-40, excluding an adenomatoid tumour of the testis.
The negative staining technique of Heine is a simple, inexpensive, and efficient way of screening for Cryptosporidium parvum and Cystoisospora belli [4].
In addition, when a positive staining for CEA and a negative staining for CAL were considered as an indication of malignancy, the sensitivity of cytological and immunocytochemical results was shown to be 97%, with 100% specificity, a positive predictive value of 100%, a negative predictive value of 95%, and an overall accuracy of 98%.
We observed a similar percentage (46.4%) of cases with negative staining in adjacent non-neoplastic mucosa.
Among these cases with a negative staining pattern, a positive serologically elevated serum tTG was seen in the four cases where it was measured.
Although pituitary adenomas can have somewhat similar morphologic features, the presence of strong and diffuse TTF-1 nuclear expression with negative staining for pituitary hormones excludes the diagnosis of a pituitary adenoma.