Neuron specific enolase (NSE) and neurofilament
immunohistochemical staining were performed, results of which showed evidence of a neuroectodermal population and intermediate filaments from neurons, respectively.
accumulate in the blood not only in Alzheimer's but also in the course of other neurodegenerative disorders.
Cerebrospinal fluid levels of chitinase 3-like 1 and neurofilament
light chain predict multiple sclerosis development and disability after optic neuritis.
Postsynaptic AMPARs, NMDARs, and nerve fibers were identified by immunostaining for GluA2 (red), GluN1 (green), and neurofilament
(NF) (grey), respectively.
light chain predicts progression in progressive supranuclear palsy.
Unstained sections were further processed for S-100 and neurofilament
immunostaining according to the manufacturer's instructions.
This was probably due to Wallerian degeneration and/or to arrested development of the corticospinal and corticobulbar tracts, as well as pontocerebellar connections, which were well demonstrated by neurofilament
immunostaining (Figure 6, C and D).
In spindle-shaped cells, S-100 showed the highest positive rate (96.6%, 144/149), followed by NSE (85.0%, 68/80), neurofilament
(69.1%, 47/68), vimentin (60.0%, 3/5), Bcl-2 (58.3%, 7/12), synaptophysin (54.4%, 31/57), CD56 (50.0%, 9/18), CD34 (33.3%, 1/3), calcitonin (20.0%, 4/20), and vasoactive intestinal peptide (13.3%, 4/30).
Homogenates of 8 mm of the spinal cord around the lesion epicenter were utilized for Western blot analysis to detect the cytoskeletal protein neurofilament
For ICC, an antibody for neurofilament
(NF) was used to stain the intermediate filaments of DRG neurons.
The structural integrity of axons was evaluated in hypoxic/ischemic animals 72 h after the hypoxia/ischemia event by analyzing the plasma levels of phosphorylated neurofilament
heavy protein (pNF-H).