Earlier we reported increased protein aggregation and formation of neurofibrillary tangles rich in hyperphosphorylated neurofilament
tau and ubiquitin and amyloid [beta] protein plaques of varied maturity in non-demented individuals (control) beyond 60 yr of age and further higher density in Alzheimer's disease (AD) in the frontal cortex and hippocampus (19).
Moreover, they argue, the length of motor neuron axons and their normal neurofilament
content, higher than other neurons, make them especially vulnerable to overproduction of neurofilaments
The tumor cells express neuronal markers, such as class III-[beta] tubulin, microtubule associated protein 2 (MAP2), NeuN, and neurofilament
These cells then cultured to induce spontaneous differentiation for 15 days were stained for the marker of each three germ layers, as evidenced by immunohistochemical staining with antibodies to neurofilament
(ectoderm, Figure 5B), afetoprotein (endoderm, Figure 5C), and cardiac troponin 1 (mesoderm, Figure 5E).
Additionally, 5-[micro]m sections were taken from the paraffin blocks and stained using antibodies against Melan-A (prediluted, monoclonal, catalog number 790-2990; Ventana Medical Systems, Tucson, Arizona), S100 protein (prediluted, polyclonal, catalog number 760-2523; Ventana Medical), and neurofilament
(prediluted, clone 2F11, monoclonal, catalog number 760-2661; Ventana Medical) following the manufacturer's protocols in a BenchMark XT automated slide stainer (Ventana Medical).
Arsenic inhibits neurofilament
transport and induces perikaryal accumulation of phosphorylated neurofilaments
: roles of JNK and GSK-3beta.
In the case of Al-maltolate treated aged rabbits, amyloid precursor protein (APP), A[beta], neurofilament
protein like unphosphorylated tau, ([alpha]--1 antichymotrypsin and ubiquitin are observed, while, in AD in addition to the above features neurofilament
protein is hyperphoshorylated (40,43).
proteins are major targets of HNE modification (54).
This finding was confirmed by immunohistochemistry, which was positive for chromogranin, synaptophysin, neuron-specific enolase, and neurofilament
(figure 1, B).
Schwann cell hamartomas express S100 protein in a strong, diffuse pattern with both nuclear and cytoplasmic staining (Figure 3, C), and some of these lesions have rare axons that stain with neurofilament
Comparison of neurite outgrowth with neurofilament
protein subunit levels in neuroblastoma cells following mercuric oxide exposure.
Immunostaining was performed with glial fibrillary acidic protein, neurofilament
protein, S-100, and the proliferation index MIB-1 (Ki-67).