nickase


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nickase

[′ni‚kās]
(biochemistry)
An enzyme that causes single-stranded breaks in duplex deoxyribonucleic acid, allowing it to unwind.
References in periodicals archive ?
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
The CRISPR/ Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.
For knockout of the chicken myostatin (MSTN) gene, the nickase target loci were designed in exon 1 of chicken MSTN gene (Figure 1A).
The Cas9-D10A nickase target sites were designed in exon 1 of chicken MSTN gene (Figure 1A).
In the MSTN knockout DF1 cells induced by the Cas9-D10A nickase, the various mutant genotypes of the mixed population were identified (Figure 2C).
In this study, we designed two adjacent (+7 bp offset, Figure 1B) but opposite single-strand breaks in chicken MSTN gene for Cas9-D10A nickase.
The red sequences are the targeted sites of the CRISPR-associated protein 9 (Cas9)-D10A nickase.
The data demonstrates strong activities versus monomeric wild-type Cas9 nucleases and nickases, as well as increased genomic specificities with no evidence of unwanted mutagenesis at off-target sites.
The specificity of fCas9 was shown to be at least four-fold higher than that of paired nickases at human genome loci with highly similar off-target sites elsewhere in the genome.