nickase


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nickase

[′ni‚kās]
(biochemistry)
An enzyme that causes single-stranded breaks in duplex deoxyribonucleic acid, allowing it to unwind.
References in periodicals archive ?
Wang et al., "TALE nickase mediates high efficient targeted transgene integration at the human multicopy ribosomal DNA locus," Biochemical and Biophysical Research Communications, vol.
Kim et al., "Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases," Genome Research, vol.
Singhal et al., "Characterization of Staphylococcus aureus Cas9: a smaller Cas9 for all-in-one adeno-associated virus delivery and paired nickase applications," Genome Biology, vol.
[45] Shen, B., et al., Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Plasmid Construction and Gene Targeting Mediated by CRISPR/Cas9 Nickase. To create the donor vector, cassettes consisting of marker genes NeoR, EGFP, and HSVtk and inverted loxP sequences were constructed into the EcoRI-BamHI site of pBluescript SK.
The CRISPR/ Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.
The Cas9D10A, mutant CRISPR Nickase, has been produced to selectively make a single-strand DNA cut at the target sequence.
Nickase, which is a mutated Cas9 (Cas9-D10A) enzyme, was newly developed for precise genomic modification [12].
Cas9 nickase variants (Cas9-D10A or Cas9-H840A) containing a single inactive nuclease domain cleave only one DNA strand to create a single-strand break at the target sites.
The molecular biology grade ethanol has no detectable Dnase, Rnase or Nickase activity.