Keywords: Acrylates, Azomethineylide, 1,4-Naphthoquinone, Ninhydrin
, Proline, Pyrrolizidine.
The method  is based on ion-exchange chromatography with post-column derivatization using ninhydrin
and adopted from the manufacturer: The injection volume was 20 [micro]l.
The results of the ninhydrin
test indicated that aphids in the pogostone treatment groups produced smaller amounts of honeydew (F = 38.
Amino acids were separated by IE-HPLC using a lithium high-performance physiological column (Biochrom Ltd) followed by postcolumn derivatization of the eluting amino acids with ninhydrin
and detection of the derivatized amino acids at 570 nm and 440 nm (33).
Serum cysteine and cystine were measured using an adaption of the Gaitonde procedure  developed for the detection of amino acids which uses the colorimetric reaction of the amino acids with ninhydrin
Then 2 cm3 of the supernatant of acid ninhydrin
Proline is coupled with ninhydrin
to form a colored complex.
The determination of total alkaloids is based on the reaction with ninhydrin
, a strong oxidative agent that reacts with amino acids and yields a violet-blue product, which has the maximum absorption at 570 nm.
The degree of crosslinking was determined using the ninhydrin
assay as previously described .
56, Method 1--Post-Column Ninhydrin
Derivatisation, which was rolled out in January 2014.
Estimation of Free amino group concentration: Ninhydrin
assay was used to determine the free amino group concentration of each test sample as per the procedure of Sung et al.
It can be with a 254 nm UV light on a fluorescent plate (most plates should be ordered with the F indicator, as it is called) or some other reagent that can be sprayed on (as a ninhydrin
solution to detect amino acids).