ninhydrin


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Related to ninhydrin: Ninhydrin test

ninhydrin

[nin′hī·drən]
(organic chemistry)
C9H4O3·H2O White crystals or powder with a melting point of 240-245°C; soluble in water and alcohol; used for the detection and assay of peptides, amines, amino acids, and amino sugars. Also known as triketohydrindene hydrate.
References in periodicals archive ?
The method [19] is based on ion-exchange chromatography with post-column derivatization using ninhydrin and adopted from the manufacturer: The injection volume was 20 [micro]l.
The results of the ninhydrin test indicated that aphids in the pogostone treatment groups produced smaller amounts of honeydew (F = 38.
Amino acids were separated by IE-HPLC using a lithium high-performance physiological column (Biochrom Ltd) followed by postcolumn derivatization of the eluting amino acids with ninhydrin and detection of the derivatized amino acids at 570 nm and 440 nm (33).
Serum cysteine and cystine were measured using an adaption of the Gaitonde procedure [10] developed for the detection of amino acids which uses the colorimetric reaction of the amino acids with ninhydrin.
The determination of total alkaloids is based on the reaction with ninhydrin, a strong oxidative agent that reacts with amino acids and yields a violet-blue product, which has the maximum absorption at 570 nm.
The degree of crosslinking was determined using the ninhydrin assay as previously described [10].
56, Method 1--Post-Column Ninhydrin Derivatisation, which was rolled out in January 2014.
Estimation of Free amino group concentration: Ninhydrin assay was used to determine the free amino group concentration of each test sample as per the procedure of Sung et al.
It can be with a 254 nm UV light on a fluorescent plate (most plates should be ordered with the F indicator, as it is called) or some other reagent that can be sprayed on (as a ninhydrin solution to detect amino acids).