nitrocellulose filter

nitrocellulose filter

[¦nī·trō′sel·yə‚lōs ‚fil·tər]
(cell and molecular biology)
A very thin filter whose fibers selectively bind single-stranded deoxyribonucleic acid (DNA) but not double-stranded DNA or ribonucleic acid.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
We filtered 50 mL portions of the samples recovered through a 0.2 pm pore-size nitrocellulose filter. Filters were then incubated at 42.5[degrees]C after being placed on des oxycholate agar and further differentiated on Hektoen enteric agar and MacConkey agar.
The gel was stained with Coomassie Brilliant Blue and compared with molecular weight standards (Bio-Rad, broad range) or transferred to nitrocellulose filter for Western blot analysis.
After separation, proteins were transferred to 0.45 [micro]m pore size nitrocellulose filter (100 V, for 45 min) in transblot buffer (25 mM Tris, 190mM glycine and 20% methanol).
Cleaned spicules were either mounted directly in balsam on slides or retained on nitrocellulose filters which were cleared and mounted on slides.
The following materials were purchased from the sources indicated: [2,8,5'[sup.3]H]adenosine (2.3 TBq/mmol) from NEN; adenosine, AMP, ADP, ATP, cAMP, adenosine deaminase, nucleoside phosphorylase, and xanthine oxidase from Boehringer Mannheim Germany; SAH, adenine, 2'-deoxyadenosine, L-homocysteine, diadenosine diphosphate, [N.sup.6]-methyladenosine, and cGMP from Sigma; and 0.45 [micro]m nitrocellulose filters from Schleicher and Schnell.
The protein-bound radioactivity is then trapped on nitrocellulose filters and is inversely correlated to nonradioactive cystine (3).
Nitrocellulose filters were obtained from Schleicher & Schuell.
The purified TPO proteins were electro-transferred onto nitrocellulose filters (0.45 nm pore size; Paraplan, Budapest, Hungary) at 180 mA for 24 h at 4 [degrees]C.