Sixteen different combinations of plant growth regulators were used for induction and elongation of adventitious shoots from the nodal zone of shoot explants.
Differentiation of shoot buds was limited only to the nodal zone of the explants (Figure 2C) isolated from the shoots elongated in vitro from the germinated seedlings.
Code Morphological descriptor Seedling development: Establishment from seed 00 Dry seed 01 Seed imbibition 02 Radicle emerged 03 Coleoptile emerged 04 One leaf unfolded 05 Two leaves unfolded 06 Three leaves unfolded 07 Four leaves unfolded 08 Five leaves unfolded 09 Axillary bad or stolon initiation Vegetative: Establishment from sprigs and internode elongation of shoots in established 0 New shoat web no detectable nodal zones
One detectable nodal zone
Two detectable nodal zones
Nine nodal zoom Booting: Swelling of the flag leaf sheath with developing inflorescence 21 Inflorescence fills 10% of flag leaf sheath 22, etc.
The course covers element-level considerations including struts, ties, and nodal zones
, and serviceability considerations including crack control, shear stress check, and sizing of members to minimize diagonal cracking.