Dyall-Smith, "Mutations in DNA gyrase result in novobiocin
resistance in halophilic archaebacteria," Journal of Bacteriology, vol.
test is used to differentiate coagulase-negative staphylococci.
However, in the present investigation, the results of the antibiotic sensitivity test with eight different antibiotics indicate that the strain CASKS5 was found to be susceptible to the majority of antibiotics, for example, amikacin, erythromycin, novobiocin
, oxytetracycline, and vancomycin, although it was resistant to only three antibiotics, namely, amoxicillin, bacitracin, and cephalothin (Figure 2).
(UCB) isolates showed highly resistant to ampicillin, novobiocin
, penicillin (100%), kanamycin (97%), tetracycline (94%), chloramphenicol (91%), streptomycin (77%), and low levels of resistance to gentamycin, while the percentage of resistance to (UCC-D) isolates were highly resistant to ampicillin, penicillin (100%), novobiocin
(94%), tetracycline (61%), streptomycin (53%), gentamicin (50%), and low levels of resistance to kanamycin (48%), chloramphenicol (47%) (Figure 1).
The following chemicals and reagents were used: Ampicillin (FLUKA), Isopropyl AY-D-1 thiogalactopyranoside (IPTG; SIGMA), LB broth (SIGMA), XL-1 Blue competent cells (AGILENT TECHNOLOGIES UK LTD), Agar (FLUKA), 1Kb DNA ladder (NEW ENGLAND BIOLABS), PCR nucleotide mix, Taq buffer and Taq polymerase X (PROMEGA), Primers (EUROFINS MWG OPERON), GStrap FF (GE HEALTHCARE), Phenol blue (SIGMA-ALDRICH), 10X Tank buffer (BIO-RAD), N-N-N-N- tetramethylethylenediamine (TEMED) (SIGMA), Ammonium per sulphate (APS) (SIGMA), Sugar donor UDP-Glucose (Sigma X), novobiocin
, kaempferol and quercetin (SIGMA), UDP Rhamnose (a kind gift from Prof.
epidermidis, the tests described by Bannerman (23) and MAcFaddin (24) were used: production of bound coagulase factor ("clumping") and coagulase-free production of hemolysis, pyrrolidonyl arylamidase enzyme, urease, ornithine decarboxylase, novobiocin
susceptibility and fermentation of the carbohydrates mannitol, mannose and trehalose.
We observed resistance of microbes to commonly used antibiotics (novobiocin
and ampicillin), but not from another antibiotic (tetracycline) that was rarely prescribed in 2012, the year preceding our study.
Staphylococcus saprophyticus was susceptible to ampicillin, cephalexin, and trimethoprim-sulfamethoxazole and resistant to nalidixic acid and novobiocin
Ratajczak, "Modulation of chaperone function and cochaperone interaction by novobiocin
in the C-terminal domain of Hsp90: evidence that coumarin antibiotics disrupt Hsp90 dimerization," Journal of Biological Chemistry, vol.
Escherichia coli O157 samples were enriched in tryptic soy broth (TSB), exposed to an immunomagnetic separation step and then cultured on cefixime potassium tellurite sorbitol MacConkey (CT SMAC) and Rainbow agar containing novobiocin
and tellurite (NT Rainbow) as previously described (Cooley et al.
Some observations on the incorporation of novobiocin
into Hektoen enteric agar for improved Salmonellae isolation.
Nonlactating-cow therapy with a formulation of penicillin and novobiocin
: mammary irritation and residues.