We focused our analysis on the number of Iba1 expressing microglia in the glomerular layer, granular cell layer, and olfactory tract of controls and AD, PDD/DLB, and FTLD cases.
By comparing the mean diameter of Iba1+ cells in the glomerular layer, granular cell layer, and olfactory tract between controls, AD, PDD/DLB, and FTLD cases, we observed an increased diameter of Iba1+ cells in all three regions analyzed, however only reaching significance for the FTLD cases (Figures 4(a) and 4(b)).
To further delineate the level of microglial activation we analyzed the expression of CD68, labelling lysosomal glycoproteins in microglia and indicative for phagocytic activity , in the glomerular layer, granular cell layer, and olfactory tract of AD, PDD/DLB, FTLD, and control cases (Figure 5(a)).
There was no statistically significant difference between the control and the olfactory tract section groups, t = 0.34, p > 0.10.
As shown in Figure 5, no subject could reach the goal after the eye-enucleation while the subjects maintained their discrimination after the olfactory tract sections because the eye-enucleated fish ate flaked fish food and fresh bloodworms in the aquarium and survived 3 months after the experiment.
Although there were two differences between the control and olfactory tract sectioned groups (surgical opening of the skull and injury to the skin and damage to the olfactory sense), there were no significant differences between the performances of the control and olfactory tract sectioned groups.