oligonucleotide


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Oligonucleotide

A deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) sequence composed of two or more covalently linked nucleotides. Oligonucleotides are classified as deoxyribooligonucleotides or ribooligonucleotides. Fragments containing up to 50 nucleotides are generally termed oligonucleotides, and longer fragments are called polynucleotides. See Deoxyribonucleic acid (DNA), Ribonucleic acid (RNA)

A deoxyribooligonucleotide consists of a 5-carbon sugar called deoxyribose joined covalently to phosphate at the 5 and 3 carbons of this sugar to form an alternating, unbranched polymer. A ribooligonucleotide consists of a similar repeating structure where the 5-carbon sugar is ribose. Chemically synthesized oligonucleotides of predetermined sequence have proven to be very useful for studying a large number of biochemical processes. In the 1960s, these compounds were used to decipher the genetic code. Later, chemically prepared deoxyoligonucleotides were joined to form genes for transfer RNAs. Gene synthesis from synthetic deoxyoligonucleotides is now routinely used to prepare genes and modified genes for proteins having potential clinical applications. Oligonucleotides have also been used to diagnose genetic disorders and bacterial or viral infections. See Gene, Genetic code, Genetic engineering, Nucleic acid

oligonucleotide

[‚äl·ə·gō′nü·klē·ə‚tīd]
(biochemistry)
A polynucleotide of low molecular weight, consisting of less than 20 nucleotide polymers.
References in periodicals archive ?
The binding is sequence specific as shown by competition with unlabeled oligonucleotide of the same sequence.
and Rossi (Beckman Research Institute, California) provide the proceedings of the First Annual Meeting of the Oligonucleotide Therapeutics Society held by the New York Academy of Sciences and the Oligonucleotide Therapeutics Society in September of 2005 at Rockefeller U.
The oligonucleotide containing the 3'-AzC group contained the 5' PDGF-BB aptamer sequence, a [C.
The oligonucleotide RpoB1UP is the same as the upstream rpoBF 1 but with a DNA uptake sequence (in lower case) that was added at the 5' end to permit DNA transformation (7).
Large-scale DNA and RNA oligonucleotide synthesis has now gained industry importance with proven therapeutic and diagnostic applications.
This alliance offers a straightforward solution for drug developers looking to minimize the challenges and risks associated with transitioning from a research-grade vendor to an oligonucleotide development service and manufacturing company.
Specifically, by inducing a hydrogen bond within a molecular, the new reagent obtains high-reactive aminated oligonucleotide in a few minutes with improved purification efficiency.
Complement activation was first observed in monkeys (indeed, the only animal model in which it has occurred) when several monkeys treated with short bursts of high doses of antisense oligonucleotide developed an anaphylactic-like reaction with hypotension.
The announcement was in sharp contrast to Invitrogen's steady growth over the last few years In the conference call, Invitrogen said it expects sales to be off by $7 million for its oligonucleotide and service businesses in the first quarter and molecular biology sales to be off $16 million in the first quarter.
They have also examined a variety of individual and complementary oligonucleotides, along with parameters that influence site-directed mutagenesis, including oligonucleotide length.
Chemists start by synthesizing a short segment of nucleic acid called an oligonucleotide.
6,815,429, entitled "Modulation of Oligonucleotide CpG-Mediated Immune Stimulation by Positional Modification of Nucleosides;" and MK 903127, corresponding to U.