Novartis AG (Basel, Switzerland) has patented BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter operably
linked to the DNA segment; and a transgene operably
linked to a second promoter; b) a BIV packaging vector construct comprising a BIV DNA sequence fragment comprising at least a gag gene or pol gene of BIV; a promoter operably
linked to the BIV DNA fragment; and a polyadenylation sequence located downstream of the BIV DNA fragment; and c) an expression vector construct comprising a gene encoding a viral surface protein.
du Pont de Nemours and Company (Wilmington, DE) has patented four chimeric genes, a first encoding lysine-insensitive aspartokinase, which is operably
linked to a plant chloroplast transit sequence, a second encoding lysine-insensitive dihydrodipicolinic acid synthase, which is operably
linked to a plant chloroplast transit sequence, a third encoding a lysine-rich protein, and a fourth encoding a plant lysine ketoglutarate reductase, all operably
linked to plant seed-specific regulatory sequences.
Louis, MO) have patented a method for conferring tolerance to salt stress and drought stress in a monocot plant including transforming the monocot plant with an expression cassette comprising at least one ABRC unit, a minimal promoter, and a DNA molecule that increases tolerance to salt stress and drought stress in plants, wherein the at least one ABRC unit, the minimal promoter, and a DNA molecule are operably
linked together to permit expression of the DNA molecule.
The method involves delivering into the subject a genetic construct comprising a coding sequence for a human proinsulin operably
connected a promoter functional in the host cells.
The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably
linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.