The cerebellum, a layer of wrinkles, is located between the
optic lobe of the below and the front of the myelencephalon (Fig.
In cephalopods the optic glands (OG) form a pair of oval organs located at the posterior edge of the optic brain tract, above the hilum of the
optic lobes (Boycott & Young 1956).
The optic tract derived from the
optic lobe measured about 7.93 mm in length and 3.35 mm in width and located at the rostral part of it (Fig.
To obtain intact vesicles with higher purity, freshly dissected
optic lobe tissue was further fractionated by continuous glycerol density gradient centrifugation (Fig 1A).
To test the absorption and final concentration of T-817 within the cephalopod nervous system, the
optic lobes of control and fed squid were studied.
As shown in Figure 1, the veratridine-induced release was much lower than the KCl-induced release of ACh by synaptosomes isolated from cuttlefish
optic lobe. Putative voltage-gated [Na.sup.+] channels were found to be expressed in the squid
optic lobe, mostly at the second-order visual giant neurons (20, 21), suggesting an unequal distribution of [Na.sup.+] channels among different cell types in the cuttlefish
optic lobe.
Dopamine modulates synaptic activity in the
optic lobes of cuttlefish, Sepia officinalis.
A frontal filament tract connects each frontal filament to its adjacent
optic lobe neuropil [ILLUSTRATION FOR FIGURES 5, 6B OMITTED].
Squid
optic lobe (8-[[micro]meter]-thick cryosections) was used as a positive control tissue and gave positive results similar to those of Nelson et al.
In previous experiments we have shown that the synaptosomal fraction from squid
optic lobe incorporates [[.sup.35S]]methionine into protein in a reaction that is largely inhibited by cycloheximide, sensitive to the ionic composition of the medium, completely unaffected by RNase, and fully inhibited by a hypo-osmotic treatment (1).
Squid
optic lobe membranes (10-75 [[micro]gram] protein) were combined with the indicated concentrations of mono-iodo [125I]-daYFnLRFa, prepared and purified to 2200 Ci/mmole as before (Payza, 1987), in a total volume of 300 [[micro]liter] of 40 mM Pipes-Tris (pH 6.5), 1% BSA.
We have previously shown that a system of protein synthesis is present in the squid giant axon (1, 2) and, more recently, in the nerve endings of the synaptosomal fraction prepared from squid
optic lobe (3, 4).
