In our experiments, AIP in zebrafish was ubiquitously expressed in all major divisions of the brain including the olfactory bulbs, telencephalon, optic tectum, cerebellum, medulla, and hypothalamus.
Notably, frog Arc was not expressed in the optic tectum, which contrasts sharply with the intense AIP expression in zebrafish optic tectum.
Citation: Yuki Shimizu et al., "Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum
of adult zebrafish," Glia, 2018; DOI: 10.1002/glia.23311
catesbeiana antigens (Reh and Nagy, 1987; Anholt et al., 1990; Key and Akeson, 1990), a strategy we have employed here to obtain reagents that may be useful in tracking the development of the optic tectum and cerebellum in this species.
Following anesthesia, tissue containing the optic tectum and cerebellum was dissected from the crania of tadpoles and froglets and homogenized together in amphibian Ringer's solution.
By finding their optimal positions between these two gradients, retinal axons replicate in the optic tectum
the same visual pattern that their cell bodies create in the retina itself, Bonhoeffer suggests.
When we injected directly into the brain third ventricle of medium sized fish, cells in the telencephalon, optic tectum
, and occasionally in the hypothalamus (not shown) were transfected with CMV-GFP (800 ng in 2 [micro]L).