Nuclear maturation rate of mature and immature vitrified buffalo oocytes after staining with orcein
stain (Mean S.
Upon cooling to room temperature the acetic acid was replaced by lactoacetic orcein
at 40[degrees]C to stain the DNA in both gametes.
The dye, a compound called orcein
, and a related substance, called O4, bind preferentially to small amyloid aggregates that are considered to be toxic and cause neuronal dysfunction and memory impairment in Alzheimer's disease.
The radicles were then hydrolysed with 5 N HCl for 5--15 min at room temperature stained with 2% acetic orcein
Testicular follicles were squashed and stained with orcein
and observed under light microscopy (Zeiss Standard, 40X).
Thin paraffin sections (5 [micro]m) were cut and stained with Haematoxylin and Eosin (Luna, 1968) for routine fibrocellular architecture, Van Gieson's stain (Luna) for collagen fibers, Gomori's reticulin method (Luna) for reticular fibers and Weigert's resorcin fuchsin and orcein
(Luna) stain for elastic fibers.
For meiotic analysis, slides were prepared using the method of squashing the anthers and coloring with acetic orcein
2% (GUERRA & SOUZA, 2002).
Towards the end of 1970s, Tanner and associates modified the previous histochemical studies on ICC (23), and reported an increase of Orcein
+ve stainable hepatic Copper binding protein (CuBP) (24).
Light microscope observations were done on temporary preparations obtained by overflowing anther content suspended in acetic orcein
In the twentieth century, biologists found orcein
to be a useful agent for staining chromosomes, enhancing their visibility under a microscope.
The onion root tips were fixed and lactic-acetic orcein
squashes on microscope slides were made for cytogenetic study.
Chromosomes were observed by acetic orcein
stain (Guo et al.