USCs are considered to be similar to ADSCs with their in vitro ability of osteogenic
von Kossa staining was performed to show the mineralization and calcium deposition of differentiated cells as a marker of osteogenic
(22) demonstrated that miR-216a was remarkably up-regulated during osteogenic
differentiation in human adipose-derived MSCs.
Alizarin red staining was increased in MSC monolayers receiving vibration and osteogenic
media compared to control, though staining was not increased for vibrated cells that did not receive osteogenic
For example, osteogenic
differentiation can be induced in vitro by bone morphogenetic proteins (BMPs)  or by a differentiation cocktail .
To avoid the influences of medicine on osteogenic
differentiation of MSCs, all diabetic volunteers who received thiazolidinedione (rosiglitazone or pioglitazone) therapy were excluded.
For example, miR-23a significantly inhibits TNF-[alpha]-induced apoptosis in MC3T3-E1 cells (osteoblastic line) by targeting Fas, a proapoptotic protein, and promotes osteogenic
differentiation by targeting Runx2 expression in ATDC5 cells [70, 71].
MC3T3-E1 cells (3 x 104 cells) were seeded in 24-well plates containing the above Ti disc samples including polished Ti, SLA-treated Ti, and SLA + HT-treated Ti in complete growth medium and osteogenic
differentiation medium (LonzaCo.
Thus there is a great need for a stable 3-D bone model that is populated with the appropriate osteogenic
cells and is responsive to factors that regulate bone functions in vivo.
To verify osteogenic
differentiation of AD-MSCs after 21 days of cultivation in osteogenic
medium, we performed relative quantification of gene transcripts of osterix (OSX), bone sialoprotein (BSP), and osteocalcin (OC) by real time RT-PCR using the [2.sup.-delta delta CT] method.
In this process, the osteogenic
differentiation marker genes (ALP, BSP, and OCN) proliferated.
This makes it extremely promising to find out the final role of ephrinB2-EphB4 signaling in osteogenic
differentiation, bone formation, and tissue regeneration, especially in the situation of bone disorders with persistent inflammation.