Samples Catalase Test Oxidase Test
Gram staining LAB001-B Negative Negative Gram positive LAB002-C Negative Negative Gram positive LAB003-D Negative Negative Gram positive LAB004-E Negative Negative Gram positive LAB005-F Negative Negative Gram positive Table 2: Phenotypic identification of isolates using API 50 CHL Kits and API web.
* Laboratory stock culture of Pseudomonas putida ATCC 31800 (oxidase test
A spoonful of sugar -improving the sensitivity of the glucose oxidase test
strip method for detecting subclinical pulmonary aspiration of enteral feed.
Other tests used to identify the organism were oxidase test
, catalase test, carbohydrate fermentation test, and nitrate reduction test.
Provisional identification of isolates was based on Gram stain and results of oxidase test
and API 20NE identification strip (Biomerieux, Marcy l'Etoile, France).
All non-lactose fermenters were separated and subjected to oxidase test
. Oxidase negative isolates were further processed using a number of biochemical tests to confirm the isolate (Qazilbash, 2002; Zareen et al., 2014).
Identification of the isolates was done according to the conventional microbiological standard tests (Gram stain, glucose fermentation test and oxidase test
Different biochemical tests such as catalase, oxidase test
, O-F tests, IMViC tests, [H.sub.2]S production test etc.
Micrococci were identified by morphology, colony charecters and oxidase test
. All gram negative bacilli were identified by their colony charecters, smear examination, motility, oxidase test
, reaction on TSI agar, urease test, nitrate test.
rhinotracheale and Pasteurella isolate showing positive oxidase test
The cultures so obtained were subjected to Gram's staining for checking the purity of growth and morphology of organism and various biochemical tests were applied as oxidase test
, catalase test, indole test, citrate utilization, nitrate reduction and carbohydrate fermentation tests (Table 1).
To perform the oxidase test
few drops of freshly prepared oxidase reagent (0.1g tetramethyl-p-phenylene-diamine dihydrochloride in 10 ml distilled water) were poured on a piece of filter paper.