50 indicates that the kinetic of meiosis is normal at pachytene.
This study was undertaken with an aim to define criteria of normality for meiosis by assessing the frequency of meiotic prophase cell types, the frequency of pachytene substages in normal and abnormal spermatogenesis and to determine what synaptonemal complex (SC) abnormalities are found in normal and abnormal meiotic prophase and their frequencies.
Microscope analysis: Nuclei of the first meiotic prophase at leptotene, zygotene and pachytene stages were analyzed using a Zeiss Axioskop epifluorescent photomicroscope equipped with propidium iodide, fluorescein isothiocyanate, and 4,6-diamidino-2-phenyllindole filters (Carl Zeiss, MC80, Oberkochen, Germany) equipped with an epifluorescent system.
The respective frequencies of the four pachytene substages (P1, P2, P3, P4) were evaluated on 200 pachytene nuclei selected from the XY configuration, stained by BRCA1 in patients and controls; this was according to the four degrees of condensation of the sex chromosomes as previously described (1,10).
1990) and SEP1 mutants fail to pass the pachytene stage of meiosis (Tishkoff et al.
Both proteins can be found on the leptotene chromosomes, whereas only RAD51 is present at the later pachytene stage (Terasawa et al.
Though a significant positive correlation exists between the number or B-chromosomes and the number of pachytene abnormalities, e.
it was shown that temperature-induced changes of chiasma frequencies were due to failure of chromosome pairing at pachytene (Elliott, 1955).
Moderate immuno-reactivity of P450arom was found in the cytoplasm of pachytene spermatocytes (Figure 2c and d).
At this age, the testis of the mice has a high number of pachytene spermatocytes (Bellve et al.
b) Denotes pachytene spermatocytes, more mature spermatogenic cells, and spermatozoa in the testis here.