Fractions obtained by gel filtration were further investigated by paper electrophoresis
, analytical ultracentrifugation, immunoelectrophoresis in agar gels, double diffusion in agar gels, and immunotitration.
This landmark paper was published in the August 1957 issue (17) and described a method in which CA strips 20-cm long by 5-cm wide were run in a standard paper electrophoresis chamber.
In 1958, Kohn published a series of 3 papers in which he reported redesigning the paper electrophoresis chamber and reducing the size of the CA membranes (21-23).
The simple and inexpensive paper electrophoresis
method rapidly achieved popularity as a routine clinical laboratory test and led to widespread investigations of the variations of plasma proteins in disease.