In method I, HeLa cells were fixed with 2% paraformaldehyde
and permeabilized with saponin and the hybridization was performed in the presence of 5'-FITC- labeled antisense probe for intracellular detection of 18S rRNA.
Hydroxyl-containing benzoxazine monomer (Bz-OH) was synthesized in the usual manner through Mannich condensation reaction of phenol and 6-amino-1-hexanol, in the presence of paraformaldehyde
PMN were incubated with streptavidin-PE and PBMC were double labeled with streptavidin-PE and anti-CD3-PerCp during 30 min at 4[degrees]C, washed twice with PBS-EDTA and fixed with 1% paraformaldehyde
9% saline) followed by immersion in 200 ml of fixative solution that contained 4 per cent paraformaldehyde
The serum from patient 1 exhibited a diffuse fluorescence pattern that was mainly localized to the nuclei of all cells in the cochlea on both paraformaldehyde
(figure 3) and acetone (figure not included) preparations.
Sperm were fixed in 1 mL of 3% paraformaldehyde
in PBS, for 24 hours minimum at 4[degrees]C.
After 20 h of incubation, the coverslips were rinsed extensively with PBS; cells were fixed with freshly prepared 4% paraformaldehyde
in PBS for 15 min at room temperature and then rehydrated with PBS.
Sections: Ileum segments, approximately 1 cm long, were fixed in fresh 4% paraformaldehyde
solution in PBS, pH 7.
It is thought that chemical catalyst paraformaldehyde
, used by the company in its chemical blending processes, swamped a waste incinerator.
We considered vaporized hydrogen peroxide (VHP), chlorine dioxide and paraformaldehyde
pipistrellus specimen fixed by immersion in 4% paraformaldehyde
was provided by D.
Control and MK-801 groups with 6 or 24 h survival underwent either (1) transcardiac brain perfusion-fixation with 4% paraformaldehyde
for H & E, TUNEL and CM1 antibody (to active caspase-3) staining, or (2) decapitation and rapid dissection and homogenization of neocortex for DNA gel electrophoresis, Western blot analysis of active caspase3 and activity of endogenous caspasc-3-like activity (DEVD-AFC cleavage assay).