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On Day 7, porcine SCNT embryos were fixed with 4% paraformaldehyde
solution for 1 h at 4[degrees]C and then rinsed three times with PBS.
Mouse brain fixed in buffered 4% paraformaldehyde
was treated for 1 h at room temperature with formic acid (98%-100%) before being embedded in paraffin blocks (Thermo Electron, Cergy-Pontoise, France).
For devices possibly containing biological contaminants, such as biosafety cabinets, the principal investigator is asked to provide documentation of appropriate systemic disinfection, such as circulation of paraformaldehyde
We didn't realize we also needed sodium azide, paraformaldehyde
, and phosphate buffered saline.
After the treatment, the cells were washed with cold PBS, fixed with 4% paraformaldehyde
for 10 min, and permeabilized in 0.
Non-adherent cells were washed off and remaining cells were fixed with 200[micro]l of 4% paraformaldehyde
for 30 minutes.
Tenders are invited for Supply Of Propidium Iodide, Paraformaldehyde
, Dna Molecular Weight Marker, Taq Dna Polymerase, etc
The blood was replaced with normal saline (37[degrees]C) and subsequently perfusion-fixed with 4% paraformaldehyde
in PBS buffer (pH 7.
(AR grade) and aniline (AR grade) were purchased from Merck Co.
Briefly, eyes were removed and immersed in ice-cold phosphate-buffered saline (PBS), corneas were slit, and eyes were immersion fixed in buffered 4% paraformaldehyde
for 30 min (confocal microscopy) or Karnovsky's fixative (light microscopy).
After treatment, MEFs were fixed with ice-cold 30 g/L paraformaldehyde
(Sigma-Aldrich) in PBS for 15 min at room temperature.