Address : 115432, Moscow, Projected passage number
Cells were routinely cultured in RPMI 1640 medium supplemented with 0.05 mM 2-mercaptoethanol (55 mM stock, Gibco) and human serum (pooled human male AB plasma using approximately 50-70 donors per batch, Sigma-Aldrich, non-heat inactivated) to a final concentration of 10%, at 37[degrees]C, 5% C[O.sub.2], in a humidified atmosphere, to a maximum passage number
From different viewpoint, subculture of ESCs under the same condition reduces batch effect due to the difference in passage number
and at least twice subculture of the ESCs used in this study is recommended for yielding non-fluctuated data.
Increased passage number
from 4 to 9 of human adipose-derived adult stem cells increases the chondrogenic potential of cells ; whether this translates into improved benefit in vivo has yet to be addressed.
This correlated with an increase in ROS as MSC passage number
increased (Figure 2(h)).
These discrepancies may reflect different VI protocols or cells (cell lines, passage number
, etc.), species origin, sample conservation, variable virus titers in specimens, among others (RUDD & TRIMARCHI, 1989).
The original virus and passage number
4, 5, 6, 7, 8 and 9 was inoculated in group A to G with dose 0.2 mL through sub-cutaneous route (s/c), respectively.
On the other hand, an analysis of the trends of medians revealed fluctuations--both increases and decreases--up to passage number
seven, followed by a marked increase that cannot be considered as significant, probably due to the small amount of sample available in this passage.
There was room at the bottom of the page to record the session number, date, passage number
, and number of words read correctly per minute on each timed trial.
Moreover, our model improves on existing primary culture models because the latter change with passage number
and have limited lifespan.
We saw that the oxygen consumption rate can decrease when reaching a higher cell culture passage number
P- Passage number
Table 2: Activity of LDH enzyme in the cell lysates at different passages.