and Automation PerkinElmer Life and Analytical Sciences Inc.
Preliminary results using Raman spectroscopy revealed a significant peak analysis
at a Raman shift of approximately 3000 cm-1.
We therefore established standard PCR-restriction fragment length polymorphism (RFLP) methods for the 12 polymorphisms listed above and a melting peak analysis method using fluorescent probes (LightCycler assays) for the rapid detection of possibly functionally important EDN1 polymorphisms [except for +138/ex1ins/delA, for which a TaqMan assay already exists in our laboratory (11)].
Primers and restriction enzymes for PCR-RFLP methods and primers and hybridization probes for melting peak analysis (see the Data Supplement accompanying the online version of this Technical Brief at http://www.
Genotyping results obtained by PCR-RFLP analysis and by melting peak analysis with fluorescent probes correlated perfectly with DNA sequencing (Prism[TM] 310 Genetic Analyzer; Applied Biosystems).
This module offers a wizard interface that takes users through the steps of advanced peak analysis
A peak analysis
based upon a spectral library indicated that the peaks are most likely associated with benzothiazole functionality.
In addition, analysts should know how reliably they are employing a particular peak analysis
method from the uncertainties associated with operator choices such as the type of background function used in their fits.
Judging from the peak analysis
, SS2 was reacted with Surlyn about four times as much as SS1.
A pre-configured menu system allows users to select various settings including standards, test levels, test methods, modulation settings, leveling methods, limit lines, peak analysis
and failure analysis.
This collaboration will enable customers to take advantage of MD Nastran in an on demand environment to meet peak analysis
demands to ensure right-to-market and minimize time-to-market for products in highly competitive environments.