In the current study, we investigated the antioxidant capacities of malvin, oenin, ID-8, silychristin, callistephin, and pelargonin with flavonoid structures and 3,4-dihydroxy-5-methoxybenzoic acid, arachidonoyl dopamine, and 2,4,6trihydroxybenzaldehyde with phenolic structures (Figure 1) by assaying, [Fe.sup.3+] and [Cu.sup.2+] reduction activity, metal chelating activity, and [O.sub.2.sup.*-], [ABTS.sup.*+], [DPPH.sup.*], and [DMPD.sup.*+] radical scavenging capacity.
In addition, malvin, pelargonin, and silychristin exhibited inhibition values of 95.16%, 93.93%, and 95.45%, respectively, showing better lipid peroxidation inhibitory activity than the reference antioxidants BHT, [alpha]-tocopherol, and trolox.
In our study, ID-8, malvin, arachidonoyl dopamine, and pelargonin exhibited higher [Fe.sup.2+] chelating activity than reference antioxidants and other phenolic and flavonoid compounds did at 10 [micro]g/mL by chelating metal ions at levels of 54.16%, 52.21%, 50.65%, and 39.58%, respectively (Table 1).
The results obtained with this method showed that 3,4-dihydroxy-5-methoxybenzoic acid and pelargonin, with [IC.sub.50] values of 11.47 and 14.13 [micro]g/mL, respectively, possessed better [O.sub.2.sup.*-] anion radical scavenging activity than the other phenolic and flavonoid compounds and the reference antioxidants BHA, BHT, [alpha]-tocopherol, and trolox did (Table 2).
In addition, 3,4-dihydroxy-5-methoxybenzoic acid, oenin, silychristin, pelargonin, ID-8, and malvin exhibited [IC.sub.50] values of 6.05, 6.60, 6.71, 6.71, 6.80, and 7.20 ([micro]g/mL), respectively.