The design and synthesis of the gene specific primers and fluorogenic probes for Perforin
(ID: Hs 00169473_m1; GenBank reference: 5551; also listed as PRF1) and TIM-3 (ID: Hs 00262170_m1; GenBank reference: 84868; also listed as HAVCR2) mRNA were made by TaqMan[R] Gene Expression Assays (Applied Biosystems, USA) and had already been tested and validated previously by the manufacturer.
mediates endothelial cell death and resultant transplant vascular disease in cardiac allografts," The American Journal of Pathology, vol.
Lymphoma cells exhibit positive expression of cytotoxic granule proteins such as granzyme B, TIA-1, and perforin
. Other markers such as CD43 and CD45RO may be positive; positive expression of CD30 may also be found in some cases [1-4].
The process of cell death through apoptosis in NK cells activates the caspase through perforin
and granzyme B.
The proposed pathophysiological mechanism includes keratinocyte apoptosis receptor fas (CD95) or cytotoxic release of perforin
and granzyme B causing cell lysis.
Speaking from his office, in front of a "We the People" poster with an illustration of a woman wearing a headscarf, he explains that it's his responsibility to help his congregants perforin
the mitzvah of saving a life.
In addition, these CD4+ cytotoxic T cells secrete granzyme B and perforin
, previously thought to be released mainly by natural killer T cells.
On immunohistochemistry, the tumor cells were positive for CD3, CD8, perforin
, and granzyme and were negative for CD20, CD4, and CD56 (Figure 2b, 2c).
Activated CD8+ T-lymphocytes infiltrating muscle tissue can release perforin
and granzyme, causing muscle degeneration and necrosis.
was negative and granzyme was focal positive.
Flow cytometry (FCM) analysis of the BM aspirate cells using the EuroFlow lymphoid screening tube (LST) and antibody panel for NK cell chronic lymphoproliferative diseases (NK-CLPD) , complemented with other cell surface markers, showed that the neoplastic cells were positive for CD45 (high), CD2, CD26, CD38, CD94 (high), and HLADR (high, heterogeneous) antigens, and negative for surface CD3, TCR, CD4, CD5, CD7, CD8, CD11b, CD11c, CD16, CD56, CD57, and CD161, as well as for cytoplasmic CD3; in addition, they express intracellular granzyme B and perforin
[alpha]GalCer-stimulated pregnancy loss was associated with perforin
when given early.