The distribution of staining of BM89 and SVP-38 in normal CNS was neuron specific (data not shown), exhibiting predominantly neuropil and perisomatic patterns, as previously described. Perikaryal
localization of BM89 was also detected in subpopulations of rhombencephalic neurons.
Arsenic inhibits neurofilament transport and induces perikaryal
accumulation of phosphorylated neurofilaments: roles of JNK and GSK-3beta.
1C), with an outer layer of cell bodies (perikaryal
layer) enveloping an internal neuropil (Graziadei, 1971).
Immunohistochemically, the cells with ganglionic features showed focal perikaryal
staining for synaptophysin and chromogranin, whereas many of the spindled cells were immunoreactive for GFAE Immunoreactivity for MIB-1 was less than 2% of tumor nuclei, whereas that for p53 was close to 40%.
Ultrastructures of neuronal perikaryal
cytoplasm in conventional TEM (a), and embedment-free section TEM (b).
Such localization mechanisms may account for the perikaryal and axonal differences in phosphorylation activity characteristic of the squid giant fiber system.
Since kinesin is a motor responsible for the fast transport of NFs (Yabe et al., 1999), such indirect effects on motors by deregulated proline-directed kinases may contribute to the perikaryal accumulation of aggregates by blocking axon transport.
In contrast, neuronal loss accompanied by perikaryal
swelling, another archetypical injury response (Roy et al.
This method provides information on even minute neuronal structures, such as recessed cilia on sensory cells and short perikaryal
cilia on ganglion cells, which are important in categorizing the cells into groups.