The insert (huscFv) was isolated and subcloned into the pET22b expression vector containing pelB leader sequence to facilitate expression of recombinant protein in the periplasmic
Incorporation of heterologous outer membrane and periplasmic
proteins into Escherichia coli outer membrane vesicles.
The acyl chains are directed toward the periplasm suggesting that flipping occurs after a conformational change to open the periplasmic
side of MsbA; however, examination of the hydrophobicity of MsbA (Figure 14(b)) suggests that the requisite translation has already occurred with the acyl chains already extending to the periplasmic
leaflet of the inner membrane .
Cooksey, "Copper resistance in Pseudomonas syringae mediated by periplasmic
and outer membrane proteins," Proceedings of the National Acadamy of Sciences of the United States of America, vol.
Lee, "Crystal structure of a putative oligopeptide-binding periplasmic
protein from a hyperthermophile," Extremophiles, vol.
Efficient large scale purification from periplasmic
inclusion bodies in E.
Salyers, "Biochemical evidence that starch breakdown by Bacteroides thetaiotaomicron involves outer membrane starch-binding sites and periplasmic
starchdegrading enzymes," Journal of Bacteriology, vol.
Che, "Biosynthesis of silver nanoparticles from silver(I) reduction by the periplasmic
nitrate reductase c-type cytochrome subunit NapC in a silver-resistant E.
Bacteria are able to withstand certain silver concentrations and reduce them to form nanocrystals in the periplasmic
space as a defense (Klaus-Joerger et al., 2001).
Also, the enzymes in the periplasmic
space are capable of breaking down the molecules introduced from outside [27, 28].
pallidum is covered with the outer membrane (OM), periplasmic
space with endoflagella, peptidoglycan layer, and inner membrane (IM), which surrounds a cytoplasmic cylinder .
trachomatis reticulate bodies had abnormal morphology with expanded periplasmic
space or disrupted structure (Figures 5(c) and 5(d)).