The HEK293-FT cell line was selected as a standard permissive cell
line for its high transfection rate (20), (24)
2 in Basic Virology , illustrating the replication cycle of the closely related SV40 in a permissive cell
, shows no viral integration but provides no references to associated experimental evidence.
The higher load and longer detection of Zika virus RNA in this compartment might be attributable to Zika virus replication in permissive cells
of the skin (e.
After following virus growth in all permissive cells
for another 20 h, we harvested supernatants and, to confirm the production of infectious virus particles, we titrated the supernatants by using a plaque assay in Vero cells.