Role of the Brucella suis lipopolysaccharide O antigen in phagosomal
genesis and in inhibition of phagosome-lysosome fusion in murine macrophages.
After microbial infection or ligation of TLR2, Rubicon interacts with the p22phox subunit of the NADPH oxidase complex, accelerating its phagosomal
trafficking to induce a burst of ROS and inflammatory cytokines.
Wasmeier et al., "Rab27a regulates phagosomal
pH and NADPH oxidase recruitment to dendritic cell phagosomes," Nature Cell Biology, vol.
Several possible mechanisms have been proposed, including mitochondrial reactive oxygen species production, phagosomal
or endosomal rupture, and cell membrane disturbances.
A comparative analysis of maturation of phagolysosomes by acidification, recruitment of hydrolases, and degradation of phagosomal
content between the two species was performed; this sequence of events is crucial for the ability to ingest and kill microorganisms and host cells.
Nusslein-Volhard, "Live imaging of neuronal degradation by microglia reveals a role for v0-ATPase a1 in phagosomal
fusion in vivo," Cell, vol.
Schnappinger D, Ehrt S, Voskuil M, Liu Y, Mangan J, Monaban I, Dolganov G, Efron B, Butcher P, Nathan C, Schoolnik G (2003) Transcriptional adaptation of Mycobacterium tuberculosis within macrophages: Insights into the phagosomal
Further, TM4 is known to play an important role in positioning the NRAMP1 protein within the phagosomal
membrane (Malo et al., 1994).
Metal transporters, NRMP-2 and DMT-1 transport the recovered iron through the phagosomal
membrane to the cytoplasm to be stored in the macrophage bound to ferritin.
The methods in autophagy will allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes, while the methods devoted to phagocytosis will provide researchers with several modern techniques for in vivo and in vitro studies of phagosomal