Phase-Contrast Microscopy


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Phase-Contrast Microscopy

 

a research technique in microscopy that uses special equipment to produce high-contrast images of colorless, transparent objects with different structure densities, such as living microoganisms and tissue cultures.

References in periodicals archive ?
Lack of 100% correlation between the vaginal biocoenosis test and cytological result according to the Bethesda system means that assessment of vaginal microflora in phase-contrast microscopy should not be abandoned.
The approaches proposed in [14-16] were originally validated on another phase-contrast microscopy image dataset named C3H10T1/2, therefore we implemented the approaches and then applied them to the C2C12 dataset.
[2] S Huh, DF Ker, R Bise, M Chen, and T Kanade, "Automated mitosis detection of stem cell populations in phase-contrast microscopy images," IEEE Transactions on Medical Imaging, vol.
After the exposure of cultured rat cortical neuronal cultures to 35 [micro]M Glu or NMDA for 20 min, acute neuronal swelling, breakage in dendrocytes, and the indistinct nuclear shape were observed by phase-contrast microscopy (Figure 1).
extract + 350 [micro]M Glu, the neurons showed regular and distinct shape under phase-contrast microscopy unlike in 350 [micro]M Glu culture.
Figure 5a and b show the micrographs which were obtained by the polarizing microscopy and the phase-contrast microscopy of quite same cross sections of same H-Q sheet, respectively.
Figure 13 shows the micrographs obtained by phase-contrast microscopy of cross section of PP sheet cooled under different conditions (a) dry ice/ethyl alcohol, (b) ice water, and (c) 40[degrees]C water after melted on the hot stage.
In a laboratory of nephrology, however, where samples have a strong preselection, such an algorithm is not applicable and all samples must be analyzed by phase-contrast microscopy. Nonetheless, the use of the automated procedure may help to save time on red and white cell counts, thus allowing the operators to dedicate more time to the morphologic definitions.
The oocysts cannot be detected by standard ova and parasite testing; methods specific for Cyclospora include modified acid-fast or other stains, autofluorescence with ultraviolet epifluorescence microscopy, and wet mount under phase-contrast microscopy (3).
The only subtle difference is that the Mg-actin bundles tend to reach a larger size than PEG-actin bundles, and the difference is more apparent by phase-contrast microscopy (data not shown).
Depeursinge, "Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms," Applied Optics, vol.
After 20 [micro]L of Alcian blue-pyronin B supravital stain was added to the urine (13), 1-[micro]L volumes were counted in disposable chambers (Fast-Read 10; Bio-Sigma) by both brightfield and phase-contrast microscopy (Nikon Eclipse E400; Nikon Europe B.V.).

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