The xyr1 recomplementation strain was constructed as: the phleomycin resistance gene (ble)  was used as a selection marker.
We also constructed an xyr1 recomplementation strain (xyr1rec) by replacing the hygromycin B resistance gene with a phleomycin resistance gene and the xyr1 gene in [DELTA]xyr1 (Table S1; Fig.
Aminoglycoside antibiotics phleomycin
, bleomycin and related families are wide-spectrum antibiotics containing the ring pyrimidine ring .
From each dilution, 5 [micro]L was spotted onto YPD agar containing methyl methanesulfonate (MMS) (0.05%), hydroxyurea (100 mM), phleomycin
(5 [micro]g/mL), benomyl (12 [micro]g/mL), and 4-nitroquinoline (4-NQO) (0.2, 0.5, and 1.0 [micro]g/mL) or exposed to 10 or 25 J/[m.sup.2] of UV radiation in a Stratalinker model 1800 (Stratagene).
The following antibiotics were used at the indicated concentration for the selection of transfectants: 50mg/mL hygromycin B (Roche), 25mg/mL G418 (Invitrogen), 10mg/mL phleomycin
(InvivoGen), and 50mg/mL puromycin dihydrochloride (Calbiochem).