phosphate buffer


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phosphate buffer

[′fä‚sfāt ′bəf·ər]
(analytical chemistry)
Laboratory pH reference solution made of KH2PO4 and Na2HPO4; when 0.025 molal (equimolal of the potassium and sodium salts), the pH is 6.865 at 25°C.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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pH Stability: 200 uL free or 0.317 g immobilized AOG were incubated in sodium phosphate buffer solutions at various pH (3.0-8.0) at room temperature for 60 minutes and the retained activities were determined under standard assay conditions (Aslan and Tanriseven, 2007).
The electrodes were washed in phosphate buffer and dried with ultrapure nitrogen.
A complete separation was achieved with a mobile phase consisting of methanol: phosphate buffer 0.05 M (60:40, v/v) pH 5.
The cells were subsequently washed with phosphate buffered solution (PBS) and incubated with FITC-pGH (100 ng/mL) for different durations.
The cyclic voltammetry measurements were done in 0.1 M phosphate buffer (pH = 7.5) and phosphate buffer containing different amounts of NaCl.
Finally the SPE with immobilized laccase on the spheres was immersed in 3 mL of 0.05 M phosphate buffer solution at pH 5 for 30 min.
The degradation studies of HMW copolymers S1-S3 showed high stability in phosphate buffer at pH 5.0; at this pH, the HMW copolymer was nondegradable because the ester bonds in the dendritic core are stable in slightly acidic conditions.
Banana pulp tissues (0.5 g) were extracted for 10 min at 4[degrees]C with 2.5 mL of 0.05 M sodium phosphate buffer (pH 7.8) containing 0.1% (w/v) polyvinyl pyrrolidone.
In order to improve the assay reproducibility, we used the electrophoretic conditions of our previous CE assay for the measurement of other small basic analytes, in which good separations were reached by using the BTP phosphate buffer as an effective run buffer [29].
The animals of the 5th group were administered only crystalline trypsin (trypsin action control), the 6th group got phosphate buffer with the virus diluted, inhibitor and trypsin (control of reagents).
Microspheres equivalent to 50.0 mg of the drug were taken and the amount of drug entrapped was estimated by dispersing the crushed microspheres in 100.0 mL of phosphate buffer (pH 7.4) by shaking on a mechanical shaker (Remi Motors, Mumbai, India) for 12 hr.