Hexokinase and phosphoglucoisomerase
activity of aortic and pulmonary arterial tissue in individuals of various aged.
In order to estimate genetic relatedness among workers in multiple queen nests, we screened 8 random workers for polymorphism at 7 enzyme loci and in 2 buffer systems (HAD, 3-hydroxybutyrate dehydrogenase, EC 22.214.171.124; PGM, phosphoglucomutase, EC 126.96.36.199; IDH, isocitrate dehydrogenase, EC 188.8.131.52; PGI, phosphoglucoisomerase
, EC 184.108.40.206; aGDH, alpha-glycrophospahte dehydrogenase, EC 220.127.116.11; HBDH, hydroxybutyrate dehydrogenase, EC 18.104.22.168; GOT, glutamic-oxaloacetic transaminase, EC 22.214.171.124).
Peroxidase (Per1, Per2) and Phosphoglucoisomerase
(Pgi) in buffer system pH 8.2 (Poulik, 1957) was run at a constant current of 20 mA for about 6 h.
The hexokinase and phosphoglucoisomerase
activities of aortic and pulmonary artery tissue in individuals of various ages.
HCI buffer (H) pH, 7.0, Continuous tried Tris Citrate-I (CTC-I) pH 8.0, Continuous Tris Citrate-II (CTC-II) pH 7.0, Tris-Borate-EDTA (TBE) pH 8.5 4 Enzymes tried Esterase (EST), Glucose-6-phosphate dehydrogenase (G6PDH), Malate dehydrogenase (MDH), Malic enzyme (ME), 6-phosphogluconate dehydrogenase (6PGDH), phosphoglucoisomerase
(PGI), phosphoglucomutase (PGM), menadione reductase (MR), shikimic acid dehydrogenase (SKDH).
(1995) studied the isozyme systems: isocitrate dehydrogenase (IDH), phosphoglucoisomerase
(PGI), phosphoglucomutase (PGM), shikimate dehydrogenase (SKDH), 6-phosphogluconate dehydrogenase (6-PGD), leucine aminopeptidase (LAP) and malate dehydrogenase (MDH) in a total of 36 sweet, sour and ground cherries, verifying that this technique was efficient in detect polymorphism among them.
We used buffer system 8, as modified by Haufler (1985), to resolve phosphoglucoisomerase
(PGI) and triosephosphate isomerase (TPI) ; and system 9 to resolve aconitase (ACN), fructose-l,6-diphosphatase (F16DP), phosphoglucomutase (PGM) and shikimate dehydrogenase (SKDH).
The following four enzymes were assayed for all maternal families: phosphoglucoisomerase
(PGI), 6-phosphogluconate dehydrogenase (6-PGD), phosphoglucomutase (PGM), and shikimate dehydrogenase (SKD).
For example, Watt and his coworkers (1983) found that allozymes of phosphoglucoisomerase
(PGI) influence temperature tolerance in Colias butterflies.
High glucose concentration non-enzymatically glycates phosphoglucoisomerase
and inhibits the proportion of glucose 6-phosphate metabolized via the glycolytic pathway.
The enzymes assayed were acid phosphatase (Acp), aspartate aminotransferase (Aat), [Beta]-esterase ([Beta]-Est), NADP-dependent isocitrate dehydrogenase (Idh), glyceraldehyde-3-phosphate dehydrogenase (G3pd), phosphoglucoisomerase
(Pgi), and phosphoglucomutase (Pgm).
Niche partitioning between two phosphoglucoisomerase
genotypes in Amaranthus retroflexus.