Other tests look for carcinoembryonic antigens and phosphohexose isomerase
(an enzyme implicated in metastasis).
Phosphorylated [1-[sup.14]C]-2deoxy-glucose is not a substrate for phosphohexose isomerase
, so it accumulates and is not utilized by the muscle, and it is frequently measured as an index of muscle glucose uptake [26-28].
Of the enzyme systems initially assayed, alcohol dehydrogenase (ADH); lactate dehydrogenase (LDH); malate dehydrogenase (MDH); phosphohexose isomerase
(PHI/PGI); and 6-phosphogluconate dehydrogenase (6-PGD) resolved consistently.
PGA was derived from an a-amylase binding protein and PGI was derived from phosphohexose isomerase
- Electrophoretic analysis was successfully performed for nine enzyme systems: isocitrate dehydrogenase, malate dehydrogenase, malic enzyme, acid phosphatase, hexokinase, mannose-phosphate-isomerase, phosphohexose isomerase
, phosphoglucomutase, and [Alpha]-glycerophosphate dehydrogenase, (Table 4).