Incorporating glyceryl ethers into the polar head of phospholipids in the presence of a food-grade phospholipase D makes it possible to obtain new bioactive ingredients.
The researchers also optimized the percentage of phospholipase D and the molar concentration of the reactants.
DAG, diacylglycerol; DGK, diacylglycerol kinase; PLD, phospholipase D
. (B) Biosynthesis and degradation of PAF.
phospholipase A (PLA), phospholipase B (PLB), phospholipase C(PLC) and phospholipase D
Phospholipase D (PLD, EC 126.96.36.199) is a lipolytic enzyme which has gradually emerged, over recent decades, as one of the key components of a broad range of cellular and physiological processes (see  for review).
Brown, "Phospholipase D: enzymology, functionality, and chemical modulation," Chemical Reviews, vol.
(PLD) (EC 188.8.131.52) is an important enzyme that initiates membrane phospholipid degradation during ripening, senescence, and signal transduction that takes place in response to hormones and environmental stress [3, 4].
The second group comprises phospholipase C (PLC) which cleaves the glycerol-phosphate bond, and phospholipase D
(PLD), which liberates phosphatidic acid and alcohol (Figure 1).
(PLD) releases the head group from phosphatidic acid; phospholipase C cleaves the head group and the sn-3 phosphate from diacylglycerol (DAG), which usually is rapidly phosphorylated by DAG-kinase (Lundberg and Sommarin, 1992) to form phosphatidic acid.
Choline is an indicator of phospholipase D
activity, which also generates potent platelet activators such as phosphatidic acid and lysophosphatidic acid.
Abbreviations: PLA: phospholipase A; PLB: phospholipase B; PLC: phospholipase C; PLD: phospholipase D
; LPL: lysophospholipase; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PS: phosphatidylserine; PI: phosphatidylinositol; PA: phosphatidic acid; GPC: glycerophosphocholine; TMS: trimethylsilyl.