In order to perform PRAP assays on the samples, each dilution was mixed 10% phosphomolybdic acid
solution in ethanol (w/v) .
Differentiate in Stain in Ponceau de phosphomolybdic acid
1%: xylidine 1%: 1-5 min.
The Folin-Denis reagent was prepared by mixing 100 g sodium tungstate, 20 g phosphomolybdic acid
and 50 ml phosphoric acid with 750 ml water.
USA); and phosphomolybdic acid was obtained from BDH, Dorset, UK.
An equal volume of each extract and 2% solution of phosphomolybdic acid in absolute ethanol were mixed at room temperature, and the absorbance was measured by a spectrophotometer (UNICAM UV 300) at 492 nm after 5 min (Attard and Scicluna-Spiteri, 2001).
The latter is not possible if the total phenolic compounds are quantified with the Folin-Ciocalteu reagent, as this method is based on the reduction of the phosphomolybdic acid by the phenolic compounds and therefore, other reducing compounds could be detected.
Hemicelluloses are soluble in strong alkaline solutions and posses a reducing end group that could react with the phosphomolybdic acid of the Folin-Ciocalteu reagent.
The inorganic phosphorus concentration of serum was measured using the phosphomolybdic acid
method (Fiske and Subbarow, 1925).
Potassium hydroxide was from Chemapol, Checkoslovakia; manganese(II)chloride, sulphuric acid, and phosphomolybdic acid
were from Reakhim, Russia.
Tested for Presence/ Test performed absence * Phenols +++ Phosphomolybdic acid
test Tannins +++ Braemer's test Steroids and ++ Liebermann- Terpenoids (Terpenoids) Burchardt test Alkaloids - Dragendorff's test Anthraquinones - Borntrager test Flavonoids ++ Shinoda's test * - absent + Traces +++ Abundant Table 2 TLC finger printing profile of methanolic extract of H.
The literature review shows that direct transformation of epoxides to 1,2-diacetates has been obtained in the presence of various catalysts/reagents such as HCl/ZnCl2 , BF3 Et2O , DBU/LiCl , n-Bu4NCl , n- Bu4NOAc , Bu3P , LiClO4 , Er(OTf)3 , HY zeolite , (NH4)3PMo12O40 , (TBA)4P- FeW11O39 3H2O , ZrO(OTf)2 , NaBH4 , phosphomolybdic acid
or its supported on silica gel  and molecular sieves 4A .
In Folin-Ciocalteu method, reduced molecules are oxidized by a mixture of oxidants phosphotungstic and phosphomolybdic acids