Phosphotransferase


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Phosphotransferase

 

any of a subclass of transferase enzymes that catalyze the reaction for the transfer of a free or substituted phosphate in living cells. (Research on the mechanism involved in the enzyme-catalyzed transfer of phosphate groups has established that it is not the phosphate group

but rather the phosphoryl group

that is transferred; however, the expression “transfer of phosphate groups” is current in the literature.)

Phosphotransferases are classified according to the nature of the groups that accept (incorporate) the phosphate. Thus, there are alcoholic phosphotransferases (hexokinases, phosphohexokinases), carboxylic phosphotransferases (carbamate kinase, acetate kinase), nitrogenous phosphotransferases (creatine kinase, arginine kinase), and phosphoric phosphotransferases (adenylate kinase, pyrophosphokinase). Adenosine triphosphate figures in all the reactions for the transfer of phosphate. Phosphotransferases also include the enzymes that effect what is outwardly an intramolecular transfer of phosphate (phosphoglucomutase, phosphoglyceromutase); in addition, they include pyrophosphatases, numerous nucleotidyltransferases, and enzymes that transfer two phosphate groups from such donors as adenosine triphosphate to two different acceptors.

Phosphotransferases are found in the tissues of all living organisms. Their biological importance derives from their role in phosphorylation reactions, which provide cells with energy-rich compounds. More than 200 phosphotransferases are known.

V. V. ZUEVSKII

References in periodicals archive ?
This paper describes the purification of low molecular weight acid phosphatase from camel heart and the resolution of two forms possessing phosphotransferase activity and some kinetic properties.
mutans through the fructose phosphotransferase system.
0]- Murashige and Skoog Basal Media; BAP- Benzylaminopurine; TDZ-Thidiazuron; 2,4-D- 2,4- Dichloro acetic acid; AtWBC19-Arabidopsis thaliana white brown complex homologs; npt II- neomycin phosphotransferase II; GUS- [beta]-glucuronidase
Additionally, the pSUPER retroviral vectors are self-inactivating and express neomycin phosphotransferase and GFP from the PGK promoter.
faecalis showing HLAR phenotype Enzyme Activity on aminoglycoside Gentamicin Streptomycin Streptomycin adenyltransferase Absent Present 3' Phosphotransferase Absent Absent 2' Phosphotransferase & 6' Present Absent acetyltransferase (acetylase) Enzyme Activity on aminoglycoside Tobramycin- Amikacin- Netilmicin Kanamycin Streptomycin adenyltransferase Absent Absent 3' Phosphotransferase Absent Present 2' Phosphotransferase & 6' Present Present acetyltransferase (acetylase) Table II.
Production of red clover transgenic for neomycin phosphotransferase II using Agrobacterium.
A commonly used antibiotic resistance marker is the neomycin phosphotransferase 2 (npt II) gene, which confers resistance to the antibiotics kanamycin and neomycin (Gasson & Burke, 2001).
Marker genes nptII (neomycin/kanamycin phosphotransferase from Tn 5, a bacterial transposon) with terminator 3'ocs (from A.
In experiments conducted by Wu and Janssen [4], removal of an untranslated leader from mRNA for the viomycin phosphotransferase (vph) gene of Streptomyces vinaceus did not prevent its heterologous translation in E.
Glucose is then actively transported across the inner membrane via the phosphoenolpyruvate: sugar phosphotransferase system (PTS), whereas maltose enters through a specific binding protein-dependent transport system.
ViraCor's CMV Antiviral Resistance test detects key mutations associated with resistance by sequencing the CMV UL97 phosphotransferase gene and the CMV UL54 DNA polymerase gene.

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